活体动物骨髓中单细胞的图像引导移植。

Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.

Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.

Sci Rep. 2017 Jun 20;7(1):3875. doi: 10.1038/s41598-017-02896-6.

Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.

单细胞移植是对其功能进行特征分析的重要方法，但标准的移植方案依赖于细胞经静脉注射后归巢至骨髓。在此，我们提出了一种将单细胞直接移植到活体小鼠骨髓中的方法。我们开发了一个光学平台，该平台将多光子显微镜与用于微创手术的激光消融单元以及用于细胞微操作的光镊集成在一起。这些工具允许进行具有高精度空间控制的图像引导单细胞移植。该平台用于递送单个造血干细胞。随着时间的推移，追踪移植细胞的植入情况，表明该技术可用于在体内研究正常和恶性干细胞。