Turcotte Raphaël, Alt Clemens, Mortensen Luke J, Lin Charles P
Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, CPZN 8238, 185 Cambridge Street, Boston, MA 02114, USA ; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, CPZN 8238, 185 Cambridge Street, Boston, MA 02114, USA.
Biomed Opt Express. 2014 Sep 12;5(10):3578-88. doi: 10.1364/BOE.5.003578. eCollection 2014 Oct 1.
The bone marrow is an important site where all blood cells are formed from hematopoietic stem cells and where hematologic malignancies such as leukemia emerge. It is also a frequent site for metastasis of solid tumors such as breast cancer and prostate cancer. Intravital microscopy is a powerful tool for studying the bone marrow with single cell and sub-cellular resolution. To improve optical access to this rich biological environment, plasma-mediated laser ablation with sub-microjoule femtosecond pulses was used to thin cortical bone. By locally removing a superficial layer of bone (local laser osteotomy), significant improvements in multiphoton imaging were observed in individual bone marrow compartments in vivo. This work demonstrates the utility of scanning laser ablation of hard tissue with sub-microjoule pulses as a preparatory step to imaging.
骨髓是所有血细胞由造血干细胞形成的重要场所，也是白血病等血液系统恶性肿瘤的发源地。它还是乳腺癌和前列腺癌等实体瘤转移的常见部位。活体显微镜检查是一种以单细胞和亚细胞分辨率研究骨髓的强大工具。为了改善对这个丰富生物环境的光学 access，使用亚微焦耳飞秒脉冲的等离子体介导激光烧蚀来减薄皮质骨。通过局部去除骨的表层（局部激光截骨术），在体内单个骨髓腔室中观察到多光子成像有显著改善。这项工作证明了用亚微焦耳脉冲扫描激光烧蚀硬组织作为成像准备步骤的实用性。
