中度分化唇鳞状细胞癌中的癌症干细胞表达肾素-血管紧张素系统的成分。
Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.
作者信息
Ram Rachna S, Brasch Helen D, Dunne Jonathan C, Davis Paul F, Tan Swee T, Itinteang Tinte
机构信息
Gillies McIndoe Research Institute, Wellington, New Zealand.
Wellington Regional Plastic, Maxillofacial and Burns Unit, Hutt Hospital, Wellington, New Zealand.
出版信息
Front Surg. 2017 Jun 6;4:30. doi: 10.3389/fsurg.2017.00030. eCollection 2017.
AIM
We investigated the expression of the renin-angiotensin system (RAS) by cancer stem cell (CSC) subpopulations we have identified in moderately differentiated lip squamous cell carcinoma (MDLSCC).
METHOD
Ten MDLSCC samples underwent 3,3-diaminobenzidine (DAB) and immunofluorescent immunohistochemical (IHC) staining for (pro)renin receptor (PRR), angiotensin-converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1), and receptor 2 (ATIIR2). NanoString analysis and Western blotting (WB) were performed on six MDLSCC samples for gene and protein expression, respectively.
RESULTS
IHC staining showed expression of PRR, ATIIR1, and ATIIR2 on cells within the tumor nests (TNs) and the stroma. ACE was localized to the microvessels within the stroma. WB detected PRR, ACE, and ATIIR2. NanoString analysis confirmed gene expression of PRR, ACE, and ATIIR1.
CONCLUSION
Components of the RAS: PRR, ATIIR1, and ATIIR2 are expressed on two CSC subpopulations in MDLSCC, one within the TNs and the other within the stroma. The endothelium of the microvessels within the stroma expresses ACE.
目的
我们研究了在中分化唇鳞状细胞癌（MDLSCC）中已鉴定出的癌症干细胞（CSC）亚群中肾素-血管紧张素系统（RAS）的表达情况。
方法
对10个MDLSCC样本进行3,3-二氨基联苯胺（DAB）和免疫荧光免疫组织化学（IHC）染色，检测（前）肾素受体（PRR）、血管紧张素转换酶（ACE）、血管紧张素II受体1（ATIIR1）和受体2（ATIIR2）。分别对6个MDLSCC样本进行NanoString分析和蛋白质印迹法（WB），以检测基因和蛋白质表达。
结果
免疫组织化学染色显示肿瘤巢（TNs）和基质内的细胞表达PRR、ATIIR1和ATIIR2。ACE定位于基质内的微血管。蛋白质印迹法检测到PRR、ACE和ATIIR2。NanoString分析证实了PRR、ACE和ATIIR1的基因表达。
结论
RAS的组分：PRR、ATIIR1和ATIIR2在MDLSCC的两个CSC亚群上表达，一个在肿瘤巢内，另一个在基质内。基质内微血管的内皮细胞表达ACE。
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