Pasin Daniel, Cawley Adam, Bidny Sergei, Fu Shanlin
Centre for Forensic Science, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
Australian Racing Forensic Laboratory, Racing NSW, Sydney, New South Wales, 2000, Australia.
Anal Bioanal Chem. 2017 Oct;409(25):5821-5836. doi: 10.1007/s00216-017-0441-4. Epub 2017 Jun 20.
The proliferation of new psychoactive substances (NPS) in recent years has resulted in the development of numerous analytical methods for the detection and identification of known and unknown NPS derivatives. High-resolution mass spectrometry (HRMS) has been identified as the method of choice for broad screening of NPS in a wide range of analytical contexts because of its ability to measure accurate masses using data-independent acquisition (DIA) techniques. Additionally, it has shown promise for non-targeted screening strategies that have been developed in order to detect and identify novel analogues without the need for certified reference materials (CRMs) or comprehensive mass spectral libraries. This paper reviews the applications of HRMS for the analysis of NPS in forensic drug chemistry and analytical toxicology. It provides an overview of the sample preparation procedures in addition to data acquisition, instrumental analysis, and data processing techniques. Furthermore, it gives an overview of the current state of non-targeted screening strategies with discussion on future directions and perspectives of this technique. Graphical Abstract Missing the bullseye - a graphical respresentation of non-targeted screening. Image courtesy of Christian Alonzo.
近年来，新型精神活性物质（NPS）的激增促使人们开发了众多用于检测和鉴定已知及未知NPS衍生物的分析方法。高分辨率质谱（HRMS）因其能够使用数据非依赖采集（DIA）技术测量精确质量数，已被确定为在广泛分析背景下对NPS进行广泛筛查的首选方法。此外，它在无需认证参考物质（CRM）或全面质谱库的情况下检测和鉴定新型类似物的非靶向筛查策略中也显示出前景。本文综述了HRMS在法医药物化学和分析毒理学中对NPS分析的应用。除了数据采集、仪器分析和数据处理技术外，还概述了样品制备程序。此外，本文还概述了非靶向筛查策略的现状，并讨论了该技术的未来方向和前景。图形摘要 未命中靶心——非靶向筛查的图形表示。图片由克里斯蒂安·阿隆索提供。
