Department of Zoology, Government College University, Faisalabad, Pakistan.
Government College Women University, Faisalabad, Pakistan.
Biol Trace Elem Res. 2018 Mar;182(1):130-139. doi: 10.1007/s12011-017-1080-5. Epub 2017 Jun 20.
Silver nanoparticles (Ag-NPs) have wide applications in the medical field; however, the toxicological effects are still poorly studied. The study was aimed to determine the effects of 15.78 nm spherical and amine-coated Ag-NPs on hematology and histology of gills and liver tissues in 28 days treated Labeo rohita (L. rohita). It was found that Ag-NPs induced alterations in the hematological parameters in a dose dependent manner. The Ag-NPs also induced histological alterations in a dose-dependent manner. In gill tissues, it induced fusion of secondary lamellae, separation of gill epithelium, fusion and necrosis of lamellar cells, hyperplasia, deformed cartilaginous skeleton, separation and lifting of epithelium, and curling of lamellae in a dose dependent manner. In the liver, Ag-NPs produced abnormalities in hepatic tissues by reducing the size of hepatocytes and nuclei, and stimulated the production of necrotic and apoptotic bodies. It was concluded that Ag-NPs are toxic to aquatic organisms and induce hematotoxicity and histopathological conditions in exposed fish.
银纳米粒子（Ag-NPs）在医学领域有广泛的应用；然而，其毒理学效应仍研究不足。本研究旨在确定 15.78nm 球形和胺基修饰的 Ag-NPs 对处理 28 天后的罗非鱼（L. rohita）鳃和肝脏组织的血液学和组织学的影响。结果发现，Ag-NPs 以剂量依赖的方式诱导血液学参数的改变。Ag-NPs 也以剂量依赖的方式引起组织学改变。在鳃组织中，它以剂量依赖的方式诱导次级鳃瓣融合、鳃上皮分离、鳃瓣细胞融合和坏死、增生、变形的软骨骨架、上皮分离和提起以及鳃瓣卷曲。在肝脏中，Ag-NPs 通过减小肝细胞和细胞核的大小对肝组织产生异常，并刺激坏死和凋亡小体的产生。研究结论认为，Ag-NPs 对水生生物有毒，会诱导暴露鱼类产生血液毒性和组织病理学变化。
