Colosi Cristina, Costantini Marco, Barbetta Andrea, Dentini Mariella
Department of Chemistry, University of Rome "La Sapienza", P.le A. Moro 5, 00185, Rome, Italy.
Methods Mol Biol. 2017;1612:369-380. doi: 10.1007/978-1-4939-7021-6_26.
3D bioprinting is an emerging field that can be described as a robotic additive biofabrication technology that has the potential to build tissues or organs. In general, bioprinting uses a computer-controlled printing device to accurately deposit cells and biomaterials into precise architectures with the goal of creating on demand organized multicellular tissue structures and eventually intra-organ vascular networks. The latter, in turn, will promote the host integration of the engineered tissue/organ in situ once implanted. Existing biofabrication techniques still lay behind this goal. Here, we describe a novel microfluidic printing head-integrated within a custom 3D bioprinter-that allows for the deposition of multimaterial and/or multicellular within a single scaffold by extruding simultaneously different bioinks or by rapidly switching between one bioink and another. The designed bioprinting method effectively moves toward the direction of creating viable tissues and organs for implantation in clinic and research in lab environments.
3D生物打印是一个新兴领域，可被描述为一种具有构建组织或器官潜力的机器人增材生物制造技术。一般来说，生物打印使用计算机控制的打印设备将细胞和生物材料精确沉积到精确的结构中，目的是按需创建有组织的多细胞组织结构，并最终构建器官内血管网络。反过来，一旦植入，后者将促进工程组织/器官在原位与宿主的整合。现有的生物制造技术仍未实现这一目标。在此，我们描述了一种集成在定制3D生物打印机内的新型微流控打印头，它能够通过同时挤出不同的生物墨水或在一种生物墨水和另一种生物墨水之间快速切换，在单个支架内沉积多种材料和/或多种细胞。所设计的生物打印方法有效地朝着为临床植入和实验室环境研究创建可行组织和器官的方向发展。
