微波消融对肝细胞癌患者T细胞亚群及细胞因子的影响。
Effects of microwave ablation on T-cell subsets and cytokines of patients with hepatocellular carcinoma.
作者信息
Zhang Haiwen, Hou Xiaowei, Cai Hongjian, Zhuang Xingjun
机构信息
a Department of Oncology , 401th Hospital of PLA , Qingdao , China.
出版信息
Minim Invasive Ther Allied Technol. 2017 Aug;26(4):207-211. doi: 10.1080/13645706.2017.1286356. Epub 2017 Feb 20.
OBJECTIVES
To investigate the effects of microwave ablation on T-lymphocyte subsets and cytokines in patients with hepatocellular carcinoma.
MATERIAL AND METHODS
Peripheral blood was collected from 45 patients with hepatocellular carcinoma treated by microwave ablation before treatment, one week and one month after treatment. T cells (CD3+, CD4+ and CD8+ cells), CD4+ CD25+ Tregs, and CD16+ CD56+ NK cells were analyzed by flow cytometry. Levels of cytokines (IL-2, IFN-γ, TNF-α, IL-12, IL-4, IL-6, IL-8, and IL-10) were determined by a Luminex 200 analyzer.
RESULTS
Compared with before treatment, CD3+ cells, CD4+ cells and IL-12 increased significantly at one month after the microwave ablation treatment, while IL-4, IL-10 decreased significantly.
CONCLUSIONS
Microwave ablation could relieve the suppression of immune function caused by tumors, promote the deviation of Th2/Th1, and improve immune dysfunction in patients with hepatocellular carcinoma.
目的
探讨微波消融对肝细胞癌患者T淋巴细胞亚群及细胞因子的影响。
材料与方法
收集45例接受微波消融治疗的肝细胞癌患者治疗前、治疗后1周及1个月的外周血。采用流式细胞术分析T细胞（CD3 +、CD4 +和CD8 +细胞）、CD4 + CD25 +调节性T细胞及CD16 + CD56 +自然杀伤细胞。采用Luminex 200分析仪检测细胞因子（IL-2、IFN-γ、TNF-α、IL-12、IL-4、IL-6、IL-8和IL-10）水平。
结果
与治疗前相比，微波消融治疗1个月后CD3 +细胞、CD4 +细胞及IL-12显著升高，而IL-4、IL-10显著降低。
结论
微波消融可缓解肿瘤所致的免疫功能抑制，促进Th2/Th1偏移，改善肝细胞癌患者的免疫功能障碍。
Zotero 插件