Department of Urology, Union Hospital, Tongji Medical College, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering and Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology (HUST) , Wuhan 430074, China.
Department of Urology, The Second Affiliated Hospital of Nanchang University , Nanchang 330006, China.
ACS Appl Mater Interfaces. 2017 Jul 19;9(28):23420-23427. doi: 10.1021/acsami.7b05639. Epub 2017 Jul 6.
We combine the telomerase extension reaction and microRNA (miRNA)-induced rolling circle amplification, followed by graphene oxide (GO) and nicking enzyme-assisted signal amplification as a method to analyze telomerase and miRNA-21 in urine samples with the following merits. First, it is a binary assay and can simultaneously output double signals that correspond to the quantities of telomerase and miRNA, respectively. Second, telomerase activity is enhanced by using a DNA molecular beacon probe to inhibit the formation of G-quadruplex. Third, background noise is decreased significantly via introduction of GO. Fourth, performance tests on about 258 urine samples demonstrate that this binary assay can distinguish between urine from bladder cancer patients, those with cystitis, and normal individuals. Finally, this strategy also shows great potential in distinguishing between muscle-invasive bladder cancers and non-muscle-invasive bladder cancers. The proposed strategy will greatly contribute to clinical decision-making and individualized treatments.
我们将端粒酶延伸反应和 microRNA（miRNA）诱导的滚环扩增相结合，然后进行氧化石墨烯（GO）和缺口酶辅助信号扩增，作为一种分析尿液样品中端粒酶和 miRNA-21 的方法，具有以下优点。首先，它是一种二元分析，可以同时输出分别对应端粒酶和 miRNA 数量的双信号。其次，通过使用 DNA 分子信标探针抑制 G-四链体的形成来增强端粒酶活性。第三，通过引入 GO 显著降低了背景噪声。第四，对约 258 个尿液样本的性能测试表明，这种二元分析可以区分膀胱癌患者、膀胱炎患者和正常人的尿液。最后，该策略在区分肌层浸润性膀胱癌和非肌层浸润性膀胱癌方面也显示出巨大的潜力。该策略将极大地有助于临床决策和个体化治疗。
