Wang Hui, Hu Zhao, Chen Li
Department of Nephrology, Qilu Hospital, Shandong University, Jinan, Shandong 250014, P.R. China.
Department of Nephrology, The Fourth Hospital of Jinan City, Jinan, Shandong 250031, P.R. China.
Oncol Lett. 2018 Oct;16(4):4223-4228. doi: 10.3892/ol.2018.9163. Epub 2018 Jul 17.
The current study aimed to evaluate the expression and specific role of miR-26a-5p in the progression of bladder cancer (BC). Reverse transcription-quantitative polymerase chain reaction analysis was performed to evaluate the level of miR-26a-5p in BC cancer and healthy controls. The present data showed that plasma miR-26a-5p was significantly increased in BC patients. Furthermore, BC tissues exhibited greater levels of miR-26a-5p compared with adjacent non-neoplastic tissues-26a-5p. Compared with BC patients at Ta-T1 stage, the level of miR-26a-5p was significantly elevated in BC patients ≥T2. BC patients at G3 stage demonstrated a higher plasma miR-26a-5p level compared with those at G1/2 stage. Receiver operating characteristic (ROC) analysis indicated that miR-26a-5p could differentiate BC patients from controls. Additionally, Kaplan-Meier analysis demonstrated that plasma miR-26a-5p negatively correlated with survival of BC patients. Dual luciferase reporter assay indicated that miR-26a-5p significantly suppressed the relative luciferase activity of pmirGLO-PTEN-3'UTR compared with the control. In conclusion, the current study indicated novel data that the levels of plasma miR-26a-5p was significantly increased in BC patients. Furthermore, the present study suggested that determination of plasma miR-26a-5p level could help to distinguish BC patients from healthy controls via targeting PTEN.
本研究旨在评估miR-26a-5p在膀胱癌（BC）进展中的表达及特定作用。采用逆转录定量聚合酶链反应分析来评估BC患者和健康对照中miR-26a-5p的水平。目前的数据显示，BC患者血浆中的miR-26a-5p显著升高。此外，与相邻的非肿瘤组织相比，BC组织中miR-26a-5p的水平更高。与Ta-T1期的BC患者相比，≥T2期的BC患者中miR-26a-5p的水平显著升高。G3期的BC患者与G1/2期的患者相比，血浆miR-26a-5p水平更高。受试者工作特征（ROC）分析表明，miR-26a-5p可区分BC患者与对照。此外，Kaplan-Meier分析表明，血浆miR-26a-5p与BC患者的生存率呈负相关。双荧光素酶报告基因检测表明，与对照相比，miR-26a-5p显著抑制了pmirGLO-PTEN-3'UTR的相对荧光素酶活性。总之，本研究表明了新的数据，即BC患者血浆中miR-26a-5p的水平显著升高。此外，本研究表明，通过靶向PTEN，测定血浆miR-26a-5p水平有助于区分BC患者与健康对照。
