Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw , Pasteura 5, 02-093 Warsaw, Poland.
Centre of New Technologies, University of Warsaw , Banacha 2c, 02-097 Warsaw, Poland.
Org Lett. 2017 Jul 7;19(13):3624-3627. doi: 10.1021/acs.orglett.7b01591. Epub 2017 Jun 21.
Commercially available 2'-O-pivaloyloxymethyl (PivOM) phosphoramidites were employed in an SPS protocol for RNA 5' azides. The utility of the N-RNAs in CuAAC and SPAAC was demonstrated by RNA 5' labeling, chemical ligation including fragment joining and cyclization, and bioconjugation. As a result, several new RNA conjugates that may be valuable tools for studies on biological events such as innate immune response (cyclic dinucleotides), post-transcriptional gene regulation (circular RNAs), or mRNA turnover (mG capped RNAs) were obtained.
商业上可获得的 2'-O-特戊酰氧甲基 (PivOM) 亚磷酰胺被用于 RNA 5'叠氮化物的 SPS 方案。通过 RNA 5'标记、包括片段连接和环化的化学连接以及生物缀合，证明了 N-RNAs 在 CuAAC 和 SPAAC 中的实用性。结果，获得了几种新的 RNA 缀合物，它们可能是研究先天免疫反应（环状二核苷酸）、转录后基因调控（环状 RNA）或 mRNA 周转（mG 帽 RNA）等生物事件的有价值的工具。
