a Laboratory Animal Center, Chinese PLA General Hospital , Beijing , PR China.
b State Key Laboratory of Kidney Diseases, Chinese PLA General Hospital , Beijing , PR China.
Anim Biotechnol. 2018 Apr 3;29(2):110-118. doi: 10.1080/10495398.2017.1322974. Epub 2017 Jun 21.
Coronary artery disease has a significant genetic predisposition, which mainly results from atherosclerosis. Miniature pig is an excellent model to investigate atherosclerosis. This study investigated whether the occurrence and development of atherosclerosis in the Wuzhishan miniature pigs (WZSPs) that were closely bred 12 generations had better consistency. The WZSPs (n = 9) were fed a high-cholesterol and high-fat diet (HCFD). After continuous feeding, 3 WZSPs each were sacrificed at 6, 8, and 12 months, respectively, and the general clinical manifestations and serological indexes were detected. The pathological changes of the major arteries and main organs were recorded. The results showed WZSPs were quite susceptible to the HCFD. At 6 months, plaque lesions appeared in the abdominal aorta and iliac artery, while at 8 months, they appeared in the coronary artery. At 12 months, atherosclerotic lesions could be found in all major arteries, while lipid core, cholesterol precipitation, and calcium deposition appeared in the most serious sites. The progression of arterial lesions and distribution of the lesions were highly consistent in the pigs. However, apparent variations in serum markers were observed. In conclusion, inbred WZSP is a good model to investigate atherosclerosis and has good predictability for the occurrence and development of the disease.
冠心病具有显著的遗传易感性，主要源于动脉粥样硬化。小型猪是研究动脉粥样硬化的优秀模型。本研究旨在探讨经过 12 代近交的五指山小型猪（WZSP）发生和发展的动脉粥样硬化是否具有更好的一致性。WZSP（n=9）喂食高胆固醇高脂肪饮食（HCFD）。连续喂养后，分别在 6、8 和 12 个月时处死 3 只 WZSP，检测其一般临床表现和血清学指标，记录主要动脉和主要器官的病理变化。结果表明，WZSP 对 HCFD 非常敏感。6 个月时，腹主动脉和髂动脉出现斑块病变，8 个月时，冠状动脉出现斑块病变。12 个月时，所有主要动脉均可发现动脉粥样硬化病变，而脂质核心、胆固醇沉淀和钙沉积在病变最严重的部位出现。猪的动脉病变进展和病变分布高度一致。然而，血清标志物的变化明显。总之，近交 WZSP 是研究动脉粥样硬化的良好模型，对疾病的发生和发展具有良好的预测性。
