Reitman J S, Mahley R W, Fry D L
Atherosclerosis. 1982 May;43(1):119-32. doi: 10.1016/0021-9150(82)90104-6.
Nine female Yucatan miniature swine, a breed not previously evaluated for their potential usefulness as a model for experimental atherosclerosis studies, were fed a high-fat, high-cholesterol diet for 10-12 months. These swine and 4 control (low-fat, low-cholesterol-fed) swine underwent a complete necropsy at the end of this period to characterize the atherosclerosis both by gross and microscopic examination. Cholesterol feeding led to elevated serum cholesterol levels and the development of accelerated atherosclerosis. Control animals on a low-cholesterol diet had little gross or microscopic atherosclerosis. All of the cholesterol-fed swine had more extensive atherosclerosis than any of the controls by gross inspection of the Sudan-stained arterial tissue. There was individual variation suggesting the interaction of factors in addition to the plasma cholesterol which determine the extent and severity of atherosclerosis. However, it was possible to show a positive correlation between hypercholesterolemia and (1) intimal thickening in the terminal abdominal aorta and mesenteric artery, and (2) increased fat deposition in the mesenteric artery. The cholesterol-induced atherosclerosis was characterized by the deposition of lipid in and around cells. Complicated atherosclerotic lesions similar to human atherosclerosis were characterized by marked animal proliferation, necrosis, cholesterol crystal deposition, and calcification. It is concluded that the Yucatan miniature swine represent an important additional animal model in which to study certain aspects of atherosclerosis.
九只雌性尤卡坦小型猪，这是一个此前未被评估其作为实验性动脉粥样硬化研究模型潜在用途的品种，被喂食高脂、高胆固醇饮食10至12个月。在此期间结束时，这些猪和4只对照（喂食低脂、低胆固醇饮食）猪接受了完整的尸检，以通过大体和显微镜检查来描述动脉粥样硬化情况。喂食胆固醇导致血清胆固醇水平升高以及加速动脉粥样硬化的发展。喂食低胆固醇饮食的对照动物几乎没有大体或显微镜下的动脉粥样硬化。通过对苏丹染色的动脉组织进行大体检查，所有喂食胆固醇的猪的动脉粥样硬化都比任何对照更广泛。存在个体差异，这表明除血浆胆固醇外，还有其他因素相互作用，决定了动脉粥样硬化的程度和严重程度。然而，有可能显示高胆固醇血症与（1）终末腹主动脉和肠系膜动脉内膜增厚，以及（2）肠系膜动脉脂肪沉积增加之间存在正相关。胆固醇诱导的动脉粥样硬化的特征是脂质在细胞内和细胞周围沉积。类似于人类动脉粥样硬化的复杂动脉粥样硬化病变的特征是明显的细胞增殖、坏死、胆固醇晶体沉积和钙化。得出的结论是，尤卡坦小型猪是研究动脉粥样硬化某些方面的重要额外动物模型。
