Pan Bing, Long Haoyuan, Yuan Ying, Zhang Haoyuan, Peng Yangyang, Zhou Dongke, Liu Chengli, Xiang Baiju, Huang Yongfu, Zhao Yongju, Zhao Zhongquan, E Guangxin
College of Animal Science and Technology, Southwest University, Chongqing 400716, China.
Chongqing Academy of Animal Sciences, Chongqing 402460, China.
Animals (Basel). 2022 Jul 19;12(14):1839. doi: 10.3390/ani12141839.
This study aimed to identify the genes related to the body size of pigs by conducting genome-wide selection analysis (GWSA). We performed a GWSA scan on 50 pigs belonging to four small-bodied pig populations (Diannan small-eared pig, Bama Xiang pig, Wuzhishan pig, and Jeju black pig from South Korea) and 124 large-bodied pigs. We used the genetic parameters of the pairwise fixation index (F) and π ratio (case/control) to screen candidate genome regions and genes related to body size. The results revealed 47,339,509 high-quality SNPs obtained from 174 individuals, while 280 interacting candidate regions were obtained from the top 1% signal windows of both parameters, along with 187 genes (e.g., , , , , and ). The results of the candidate gene (CG) annotation showed that a series of CGs (e.g., , , , , , and ) was enriched into the gene ontology terms. Moreover, molecular pathways, such as the PI3K-Akt, HIF-1, and AMPK signaling pathways, were verified to be related to body development. Overall, we identified a series of key genes that may be closely related to the body size of pigs, further elucidating the heredity basis of body shape determination in pigs and providing a theoretical reference for molecular breeding.
本研究旨在通过全基因组选择分析（GWSA）来鉴定与猪体型相关的基因。我们对属于四个小型猪群体（滇南小耳猪、巴马香猪、五指山猪以及来自韩国的济州黑猪）的50头猪和124头大型猪进行了GWSA扫描。我们使用成对固定指数（F）和π比率（病例/对照）的遗传参数来筛选与体型相关的候选基因组区域和基因。结果显示，从174个个体中获得了47,339,509个高质量单核苷酸多态性（SNP），同时从两个参数的前1%信号窗口中获得了280个相互作用的候选区域，以及187个基因（例如， ， ， ， ，和 ）。候选基因（CG）注释结果表明，一系列CG（例如， ， ， ， ， ，和 ）被富集到基因本体术语中。此外，PI3K-Akt、HIF-1和AMPK信号通路等分子途径被证实与身体发育有关。总体而言，我们鉴定出了一系列可能与猪体型密切相关的关键基因，进一步阐明了猪体型决定的遗传基础，并为分子育种提供了理论参考。
