Yuan Qin, Song Jiayin, Yang Weiwei, Wang Hongyan, Huo Qianyu, Yang Jie, Yu Xiaoxu, Liu Yunde, Xu Chen, Bao Huijing
School of Laboratory Science, Tianjin Medical University, Tianjin, China.
The Department of Clinical Laboratory, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Oncotarget. 2017 Jul 25;8(30):50015-50022. doi: 10.18632/oncotarget.18388.
CA125 has been used extensively to screen for neoplasms, especially in ovarian cancer. The serum CA125 level can be used as a better prognosis evaluation and it may dynamic monitoring the disease progression. We explored the effect of CA125 on ovarian cancer cell migration and its underlying mechanism. Transwell assays showed that exposure to 0.2 μg/ml or 0.4 μg/ml CA125 for 48 h increased migration of A2780 and OVCAR-3 ovarian cancer cells. This effect of CA125 was blocked addition of 200 ng/ml DKK-1, a Wnt pathway inhibitor. Conversely, addition of CA125 reversed the inhibitory effect of Wnt inhibition in A2780 cells pretreated with DKK-1. Examination of CA125 levels in serum from 97 ovarian cancer patients revealed no relationship between a patient's age or CA125 level currently used clinically for ovarian cancer diagnosis and metastasis. However, using receiver operating characteristic (ROC) curves, we identified a new cut-off value for the serum CA125 concentration (82.9 U/ml) that is predictive of metastasis. The area under the curve is 0.632. This new cut-off value has the potential to serve as a clinically useful indicator of metastasis in ovarian cancer patients.
CA125已被广泛用于肿瘤筛查，尤其是卵巢癌筛查。血清CA125水平可用于更好地评估预后，并且可以动态监测疾病进展。我们探究了CA125对卵巢癌细胞迁移的影响及其潜在机制。Transwell实验表明，用0.2μg/ml或0.4μg/ml CA125处理48小时可增加A2780和OVCAR-3卵巢癌细胞的迁移。加入200 ng/ml DKK-1（一种Wnt通路抑制剂）可阻断CA125的这种作用。相反，加入CA125可逆转用DKK-1预处理的A2780细胞中Wnt抑制的抑制作用。对97例卵巢癌患者血清CA125水平的检测显示，患者年龄或目前临床上用于卵巢癌诊断和转移的CA125水平之间没有关联。然而，使用受试者工作特征（ROC）曲线，我们确定了血清CA125浓度的一个新的临界值（82.9 U/ml），该值可预测转移。曲线下面积为0.632。这个新的临界值有可能作为卵巢癌患者转移的一个临床上有用的指标。
