Feng Ting-Ting, Zou Ting, Wang Xin, Zhao Wei-Feng, Qin Ai-Lan
Ting-Ting Feng, Ting Zou, Xin Wang, Wei-Feng Zhao, Ai-Lan Qin, Department of Infectious Diseases, the First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China.
World J Gastroenterol. 2017 Jun 7;23(21):3832-3838. doi: 10.3748/wjg.v23.i21.3832.
To investigate the levels, ratios, and clinical significance of T helper 17 (Th17) cells and regulatory T (Treg) cells in the peripheral blood of patients with autoimmune liver disease (AILD).
Forty-two AILD patients were included in the experimental group (group E), and 11 healthy subjects were recruited as the control group (group C). Flow cytometry was performed to determine the percentages of Th17 and Treg cells in peripheral blood lymphocytes. Furthermore, a range of biochemical indices was measured simultaneously in the blood of group E patients.
The percentage of Th17 cells and the Th17/Treg ratio were higher in group E than in group C ( < 0.01), whereas the percentage of Tregs was lower in the group E patients ( < 0.05). Patients in group E who were admitted with AILD in the active stage showed significantly higher Th17 percentages and Th17/Treg ratios than those measured in patients with AILD in remission ( < 0.05). In addition, among patients with AILD in the active stage, individuals that remained unhealed after hospitalization showed significantly higher baseline values of the Th17 percentage and the Th17/Treg ratio than those detected in patients who improved after treatment ( < 0.05). The results suggested that imbalance in the Th17/Treg ratio plays an important role in the pathogenesis and development of AILD.
A high Th17/Treg ratio appears to predict poor short-term prognosis in patients with AILD in the active stage.
探讨自身免疫性肝病（AILD）患者外周血中辅助性T细胞17（Th17）和调节性T（Treg）细胞的水平、比例及其临床意义。
将42例AILD患者纳入实验组（E组），招募11名健康受试者作为对照组（C组）。采用流式细胞术检测外周血淋巴细胞中Th17和Treg细胞的百分比。此外，同时检测E组患者血液中的一系列生化指标。
E组Th17细胞百分比和Th17/Treg比值高于C组（<0.01），而E组患者Treg细胞百分比低于C组（<0.05）。处于活动期入院的E组患者的Th17百分比和Th17/Treg比值显著高于缓解期AILD患者（<0.05）。此外，在活动期AILD患者中，住院后未治愈的患者的Th17百分比和Th17/Treg比值基线值显著高于治疗后好转的患者（<0.05）。结果表明，Th17/Treg比值失衡在AILD的发病机制和发展中起重要作用。
高Th17/Treg比值似乎预示着活动期AILD患者的短期预后不良。
