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在资源有限的环境中使用90%乙醇对听诊器进行消毒。

Use of 90% ethanol to decontaminate stethoscopes in resource limited settings.

作者信息

Raghubanshi Bijendra Raj, Sapkota Supriya, Adhikari Arjab, Dutta Aman, Bhattarai Utsuk, Bhandari Rastriyata

机构信息

KIST Medical College and Teaching Hospital, Imadol, Lalitpur, Nepal.

出版信息

Antimicrob Resist Infect Control. 2017 Jun 15;6:68. doi: 10.1186/s13756-017-0224-x. eCollection 2017.

Abstract

BACKGROUND

In developing countries like Nepal, 90% ethanol is cheap and is available in most hospitals. The unavailability of isopropyl alcohol (IPA) in these settings led us to compare the efficacy between 90% ethanol and isopropyl alcohol pads in reducing the bacterial contamination of diaphragm of stethoscope.

METHODS

A randomized blinded experimental study was carried out to determine the difference between cleaning stethoscopes with 90% ethanol and IPA. Cultures of diaphragm were taken before and after cleaning with one of the cleaning agent. Colony forming units (CFU) count and organism identification was done by a blinded investigator. CFU before and after cleaning were compared using Wilcoxon signed-rank test. Mann Whitney U test was used to compare the decrease in CFU count between the cleaning agents.

RESULTS

About 30% of the stethoscopes harbored potential pathogens. Significant reduction in CFU was observed with both IPA (Wilcoxon signed-rank test, value <0.001) and 90% ethanol (Wilcoxon signed-rank test, value <0.001). Comparing median decrease in CFU between cleaning with IPA and with 90% ethanol, no significant difference was found (Mann Whitney U test; U = 1357, value >0.05).

CONCLUSIONS

Both 90% ethanol and IPA are equally effective in decontaminating the diaphragm of stethoscope. Selection of agent should be done on the basis of cost and availability.

摘要

背景

在尼泊尔这样的发展中国家,90%的乙醇价格便宜,大多数医院都有。在这些地方无法获得异丙醇(IPA),这促使我们比较90%乙醇和异丙醇擦拭巾在减少听诊器膜片细菌污染方面的效果。

方法

进行了一项随机双盲实验研究,以确定用90%乙醇和IPA清洁听诊器之间的差异。在用其中一种清洁剂清洁前后,对膜片进行培养。由一位盲法研究者进行菌落形成单位(CFU)计数和微生物鉴定。使用Wilcoxon符号秩检验比较清洁前后的CFU。使用Mann Whitney U检验比较两种清洁剂之间CFU计数的减少情况。

结果

约30%的听诊器携带潜在病原体。使用IPA(Wilcoxon符号秩检验,P值<0.001)和90%乙醇(Wilcoxon符号秩检验,P值<0.001)均观察到CFU显著减少。比较用IPA和90%乙醇清洁后CFU的中位数减少情况,未发现显著差异(Mann Whitney U检验;U = 1357,P值>0.05)。

结论

90%乙醇和IPA在对听诊器膜片进行消毒方面同样有效。应根据成本和可获得性来选择清洁剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/5472973/34b71cd2ab2d/13756_2017_224_Fig1_HTML.jpg

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