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降低毒性并提高效率：乌头碱与甘草苷及甘草次酸对大鼠心肌细胞钙调节蛋白的调控

REDUCING TOXICITY AND INCREASING EFFICIENCY: ACONITINE WITH LIQUIRITIN AND GLYCYRRHETINIC ACID REGULATE CALCIUM REGULATORY PROTEINS IN RAT MYOCARDIAL CELL.

作者信息

Zhang Yuyan, Yu Li, Jin Weifeng, Fan Hongjing, Li Min, Zhou Tianmei, Wan Haitong, Yang Jiehong

机构信息

Zhejiang Chinese Medical University, Hangzhou 310053, P.R. China.

The Accessory Guangxing Hospital of Zhejiang Chinese Medical University, Hangzhou 310007, P.R. China.

出版信息

Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):69-79. doi: 10.21010/ajtcam.v14i4.9. eCollection 2017.

DOI:10.21010/ajtcam.v14i4.9
PMID:28638869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5471484/
Abstract

BACKGROUND

Compatibility of and is known to treat heart diseases such as heart failure and cardiac arrhythmias. This work answers the question that whether the active components (Aconitine, Liquiritin and Glycyrrhetinic Acid) of and could result in regulating intracellular calcium homeostasis and calcium cycling, and thereby verifies the therapeutic material basis.

MATERIALS AND METHODS

The myocardial cells were divided into twelve groups randomly as control group, Aconitine group, nine different dose groups that orthogonal combined with Aconitine, Liquiritin and Glycyrrhetinic Acid, and Verapamil group. The myocardial cellular survival rate and morphology were assessed. The expression of calcium regulation protein(RyR2, NCX1, DHPR-a1) in the myocardial cell by Western-blotting.

RESULTS

The results exhibited that Aconitine (120 uM) significantly damaged on myocardial cell, decreased the survival rate and expression of Na/Ca exchangers (NCX1) and dihydropteridine reducta-α1 (DHPR-a1), and increased the expression of ryanodine receptor type2 (RyR2) obviously. The compatibility groups (Aconitine, Liquiritin and Glycyrrhetinic Acid) all could against the damage on the myocardial cell by Aconitine at different levels.

CONCLUSION

Aconitine with Liquiritin and Glycyrrhetinic Acid may regulate the expression of calcium-regulated proteins to protect myocardial cells from damage.

摘要

背景

已知[具体药物名称1]与[具体药物名称2]配伍可治疗心力衰竭和心律失常等心脏病。本研究旨在回答[具体药物名称1]与[具体药物名称2]的活性成分（乌头碱、甘草苷和甘草次酸）是否能调节细胞内钙稳态和钙循环，从而验证其治疗的物质基础。

材料与方法

将心肌细胞随机分为十二组，即对照组、乌头碱组、与乌头碱、甘草苷和甘草次酸正交组合的九个不同剂量组以及维拉帕米组。评估心肌细胞的存活率和形态。采用蛋白质免疫印迹法检测心肌细胞中钙调节蛋白（RyR2、NCX1、DHPR-α1）的表达。

结果

结果显示，乌头碱（120 μM）显著损伤心肌细胞，降低钠/钙交换体（NCX1）和二氢蝶啶还原酶-α1（DHPR-α1）的存活率和表达，并明显增加兰尼碱受体2型（RyR2）的表达。配伍组（乌头碱、甘草苷和甘草次酸）均能在不同程度上对抗乌头碱对心肌细胞的损伤。

结论

乌头碱与甘草苷和甘草次酸可能通过调节钙调节蛋白的表达来保护心肌细胞免受损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/cf5990b5e41b/AJTCAM-14-69-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/029153ada005/AJTCAM-14-69-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/514b41c6c84b/AJTCAM-14-69-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/8df819dbcc68/AJTCAM-14-69-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/cf5990b5e41b/AJTCAM-14-69-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/029153ada005/AJTCAM-14-69-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/514b41c6c84b/AJTCAM-14-69-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/8df819dbcc68/AJTCAM-14-69-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05b6/5471484/cf5990b5e41b/AJTCAM-14-69-g004.jpg

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