Vink Roel G, Roumans Nadia J, van der Kolk Birgitta W, Fazelzadeh Parastoo, Boekschoten Mark V, Mariman Edwin C, van Baak Marleen A
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands.
Obesity (Silver Spring). 2017 Aug;25(8):1391-1399. doi: 10.1002/oby.21903. Epub 2017 Jun 22.
This study investigated whether diet-induced weight loss alters indices of in vivo postprandial fat uptake in adipose tissue (AT) and whether these changes are associated with weight regain in adults with overweight and obesity.
In this randomized controlled trial, 16 (6 male) individuals (BMI: 28-35 kg/m ) were randomized to either a low-calorie diet (1,250 kcal/d) for 12 weeks or a very-low-calorie diet (500 kcal/d) for 5 weeks (weight loss [WL] period) followed by a 4-week weight-stable (WS) period (together, the dietary intervention [DI] period) and a 9-month follow-up period. Arteriovenous difference measurements combined with stable isotope labeling ([U- C] palmitate) of a mixed meal were used to determine postprandial fatty acid uptake in AT.
Body weight was significantly reduced during the WL period (-8.2 ± 0.6 kg, P < 0.001), remained stable during the WS period (0.4 ± 0.3 kg, P = 0.150), and increased during follow-up (3.5 ± 0.8 kg, P = 0.001). Meal-derived in vivo fatty acid uptake dynamics across AT and expression of genes important for fatty acid uptake, storage, and release were not significantly changed during the DI period.
Subcutaneous AT does not appear prone to enhanced meal-derived fatty acid uptake after weight loss, nor were fatty acid uptake dynamics detected as related to weight regain.
本研究调查了饮食诱导的体重减轻是否会改变脂肪组织（AT）中体内餐后脂肪摄取指标，以及这些变化是否与超重和肥胖成年人的体重反弹有关。
在这项随机对照试验中，16名（6名男性）个体（BMI：28 - 35 kg/m²）被随机分为两组，一组接受为期12周的低热量饮食（1250千卡/天），另一组接受为期5周的极低热量饮食（500千卡/天）（体重减轻[WL]期），随后是为期4周的体重稳定（WS）期（合称饮食干预[DI]期）以及9个月的随访期。通过动静脉差值测量结合混合餐的稳定同位素标记（[U-¹³C]棕榈酸）来测定AT中的餐后脂肪酸摄取情况。
体重在WL期显著降低（-8.2±0.6千克，P<0.001），在WS期保持稳定（0.4±0.3千克，P = 0.150），并在随访期间增加（3.5±0.8千克，P = 0.001）。在DI期，AT中源自餐食的体内脂肪酸摄取动态以及对脂肪酸摄取、储存和释放重要的基因表达均未发生显著变化。
体重减轻后，皮下AT似乎不易出现源自餐食的脂肪酸摄取增强的情况，也未检测到脂肪酸摄取动态与体重反弹有关。
