Abdel Moneim Ahmed E
Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt
Toxicol Ind Health. 2016 Jun;32(6):1064-73. doi: 10.1177/0748233714545502. Epub 2014 Aug 21.
Treatment of rats with carbon tetrachloride (CCl4; 2 ml/kg body weight) once a week for 12 weeks caused a significant decrease in serum levels of testosterone, luteinizing hormone, and follicle-stimulating hormone. These decreases in sex hormones were reduced with Physalis peruviana L. (Cape gooseberry) juice supplementation. In addition, testicular activity of antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase suppressed with CCl4 were elevated after P. peruviana juice supplements. P. peruviana juice supplementation significantly increased the testicular glutathione and significantly decreased the level of lipid peroxidation and the nitric oxide production compared with the CCl4 group. In addition, the decline in the activity of antioxidant enzymes after CCl4 was ameliorated by P. peruviana Moreover, degeneration of germ and Leydig cells along with deformities in spermatogenesis induced after CCl4 injections were prevented with the supplementation of P. peruviana juice. Furthermore, P. peruviana juice attenuated CCl4-induced apoptosis in testes tissue by inhibition of caspase-3 activity. The results clearly demonstrate that P. peruviana juice augments the antioxidants defense mechanism against CCl4-induced reproductive toxicity and provides evidence that the juice may have a therapeutic role in free radical-mediated diseases and infertility.
每周一次用四氯化碳（CCl4；2毫升/千克体重）处理大鼠，持续12周，导致血清睾酮、黄体生成素和促卵泡激素水平显著下降。酸浆（灯笼果）汁补充剂可减轻这些性激素的下降。此外，用CCl4抑制的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽-S-转移酶等睾丸抗氧化酶活性，在补充酸浆汁后升高。与CCl4组相比，酸浆汁补充剂显著增加了睾丸谷胱甘肽含量，显著降低了脂质过氧化水平和一氧化氮生成量。此外，酸浆改善了CCl4处理后抗氧化酶活性的下降。此外，补充酸浆汁可预防CCl4注射后诱导的生殖细胞和睾丸间质细胞变性以及精子发生畸形。此外，酸浆汁通过抑制caspase-3活性减轻了CCl4诱导的睾丸组织细胞凋亡。结果清楚地表明，酸浆汁增强了抗氧化防御机制，以对抗CCl4诱导的生殖毒性，并提供了证据表明该汁可能在自由基介导的疾病和不孕症中具有治疗作用。
