Al-Olayan Ebtesam M, El-Khadragy Manal F, Metwally Dina M, Abdel Moneim Ahmed E
Zoology & Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt.
BMC Complement Altern Med. 2014 May 22;14:164. doi: 10.1186/1472-6882-14-164.
Pomegranate fruit has been extensively used as a natural medicine in many cultures. The present study was aimed at evaluating the protective effects of pomegranate (Punica granatum) juice against carbon tetrachloride (CCl4)-induced oxidative stress and testes injury in adult Wistar rats.
Twenty eight Wistar albino male rats were divided equally into 4 groups for the assessment of protective potential of pomegranate juice. Rats of group I (control) received only vehicles and had free access to food and water. Rats of groups II and IV were treated with CCl4 (2 ml/kg bwt) via the intraperitoneal route once a week for ten weeks. The pomegranate juice was supplemented via drinking water 2 weeks before and concurrent with CCl4 treatment to group IV. Group III was supplemented with pomegranate juice for twelve weeks. The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl4-induced reproductive toxicity in rats.
Pomegranate juice showed significant elevation in testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) those depleted by the injection of CCl4. Activity levels of endogenous testesticular antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and glutathione (GSH) contents were increased while lipid peroxidation (LPO) and nitric oxide (NO) were decreased with pomegranate juice. Moreover, degeneration of germ and Leydig cells along with deformities in spermatogenesis induced after CCl4 injections were restored with the treatment of pomegranate juice.
The results clearly demonstrated that pomegranate juice augments the antioxidant defense mechanism against carbon tetrachloride-induced reproductive toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases.
在许多文化中，石榴果实已被广泛用作天然药物。本研究旨在评估石榴（ Punica granatum ）汁对成年Wistar大鼠四氯化碳（CCl4）诱导的氧化应激和睾丸损伤的保护作用。
将28只Wistar白化雄性大鼠平均分为4组，以评估石榴汁的保护潜力。第I组（对照组）大鼠仅接受赋形剂，可自由获取食物和水。第II组和第IV组大鼠每周腹腔注射一次CCl4（2 ml/kg体重），持续十周。在CCl4处理前2周及处理期间，通过饮用水向第IV组补充石榴汁。第III组补充石榴汁12周。测定石榴对大鼠CCl4诱导的生殖毒性中血清性激素、氧化标志物、抗氧化酶活性和睾丸组织病理学的保护作用。
石榴汁可使因注射CCl4而降低的睾酮、黄体生成素（LH）和卵泡刺激素（FSH）显著升高。内源性睾丸抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）和谷胱甘肽还原酶（GR）的活性水平以及谷胱甘肽（GSH）含量均升高，而脂质过氧化（LPO）和一氧化氮（NO）则降低。此外，石榴汁治疗可恢复CCl4注射后诱导的生殖细胞和睾丸间质细胞变性以及精子发生畸形。
结果清楚地表明，石榴汁增强了针对四氯化碳诱导的生殖毒性的抗氧化防御机制，并提供了证据表明其在自由基介导的疾病中可能具有治疗作用。
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