Taylor J J, Young E T, Holcombe M, Kendall-Taylor P
Ann Clin Biochem. 1985 Jul;22 ( Pt 4):366-70. doi: 10.1177/000456328502200406.
The development and evaluation of a TSH radioligand receptor assay for TSH-binding-inhibiting immunoglobulins and its application to the study of patients with Graves' disease are described. Optimal conditions for the binding of 125I-TSH to solubilised thyroid membranes are defined. Polyethylene glycol precipitation was employed to prepare immunoglobulins from serum for assay and gave results comparable to ammonium sulphate precipitation. Immunoglobulins from normal serum did not interfere in the assay; preparations from patients with untreated Graves' disease gave positive results in 44 out of 56 cases. Coefficients of intra- and inter-assay variation were 7.0% and 9.8% respectively. The method is therefore not only sensitive and reproducible but is sufficiently simple and rapid for routine application.
本文描述了一种用于检测促甲状腺素结合抑制性免疫球蛋白的促甲状腺素放射性配体受体分析方法的开发与评估及其在格雷夫斯病患者研究中的应用。确定了¹²⁵I-促甲状腺素与溶解的甲状腺膜结合的最佳条件。采用聚乙二醇沉淀法从血清中制备免疫球蛋白用于检测，其结果与硫酸铵沉淀法相当。正常血清中的免疫球蛋白不干扰检测；未经治疗的格雷夫斯病患者的制剂在56例中有44例呈阳性结果。批内和批间变异系数分别为7.0%和9.8%。因此，该方法不仅灵敏且可重复，而且足够简单快速，可用于常规应用。
