Department of Chemistry, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, India.
Department of Biological Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, India.
Org Biomol Chem. 2020 Jul 29;18(29):5608-5616. doi: 10.1039/d0ob01277g.
Lipid droplets (LDs), the lipid-rich intracellular organelles, serve to regulate many physiological processes and therefore attention has been attracted towards their selective detection. We report positively solvatochromic lipophilic dyes, based on the push-pull framework containing coumarin-pyridine heterocycles for selective live-cell imaging of lipid droplets (LDs) in Cos-7 and McA-RH7777 cells at ultralow concentrations (200 nM). The fluorescent probes show a remarkable increase in fluorescence intensity with time with the hydrophobic core of the lipid droplets contributing to the observed intensity enhancement. The simple structural framework, red emission, strong Stokes shift (>80 nm), and excellent biocompatibility highlight their significance as a versatile imaging tool for studying lipid droplets (LDs).
脂滴（LDs）是富含脂质的细胞内细胞器，可调节许多生理过程，因此人们对其选择性检测引起了关注。我们报道了基于含有香豆素-吡啶杂环的推拉框架的正溶剂化显色亲脂性染料，可在超低浓度（200 nM）下选择性地对 Cos-7 和 McA-RH7777 细胞中的脂滴（LDs）进行活细胞成像。荧光探针的荧光强度随时间呈显著增加，这是由于脂滴的疏水性核心导致观察到的强度增强。简单的结构框架、红色发射、大斯托克斯位移（>80nm）和良好的生物相容性突出了它们作为研究脂滴（LDs）的通用成像工具的重要性。
