Angessa Tefera Tolera, Zhang Xiao-Qi, Zhou Gaofeng, Broughton Sue, Zhang Wenying, Li Chengdao
Western Barley Genetics Alliance, School of Veterinary and Life Sciences (VLS), Murdoch University, Murdoch, WA, Australia.
Grains Industry, Department of Agriculture and Food WA, South Perth, WA, Australia.
PLoS One. 2017 Jun 22;12(6):e0179715. doi: 10.1371/journal.pone.0179715. eCollection 2017.
A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties.
对由耐盐基因型CM72和盐敏感品种加德纳（Gairdner）产生的大麦（Hordeum vulgare L.）双单倍体（DH）群体在发芽、幼苗出土和第一片叶子完全展开生长阶段的耐盐胁迫能力进行了研究。发芽试验采用去离子水、150 mM和300 mM NaCl处理。幼苗期耐盐性试验设置了三个处理：对照、在幼苗出土和第一片叶子完全展开生长阶段添加150 mM NaCl。本研究结果揭示了发芽率和幼苗期生物量的超亲表型分离。在2H - 6H染色体上鉴定出12个数量性状基因座（QTL），每个QTL解释了10 - 25%的表型变异。位于3H染色体上176.5 cM处的一个QTL与第一片叶子完全展开生长阶段诱导的盐胁迫下的单株鲜重和单株干重以及幼苗出土时诱导的盐胁迫下的单株干重相关。在2H染色体上定位了一个在150 mM和300 mM盐胁迫下均稳定的发芽QTL，但与一个与幼苗期耐盐胁迫相关的QTL距离较远。本研究中鉴定出的QTL、相关标记和基因型在培育耐盐胁迫大麦品种中发挥着重要作用。
