HvNax3--一个控制野生大麦（Hordeum vulgare ssp. spontaneum）地上部钠排斥的位点。
HvNax3--a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum).
机构信息
Australian Center for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, Adelaide, South Australia, 5064, Australia.
出版信息
Funct Integr Genomics. 2010 May;10(2):277-91. doi: 10.1007/s10142-009-0153-8. Epub 2010 Jan 14.
Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na(+)) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na(+) excluder) attributed the control of the Na(+) exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na(+) accumulation by 10-25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F(2) and F(3) families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.
先前的工作确定野生大麦（Hordeum vulgare ssp. spontaneum）品种 CPI-71284-48 能够在盐生水培生长条件下限制 shoot 中的钠（Na(+))积累。利用 CPI-71284-48 与栽培大麦（H. vulgare ssp. vulgare）品种 Barque（中度 Na(+)排斥者 Barque-73）的杂交进行数量性状位点（QTL）分析，将 Na(+)排斥性状的控制归因于 7H 染色体短臂上的单个位点 CPI-71284-48，该位点被命名为 HvNax3。该位点使在 150 mM NaCl 中生长的植物的 shoot Na(+)积累减少了 10-25%。利用与水稻和短柄草的共线性生成的标记，以及对导入系和 F(2)和 F(3)群体的分析，将 HvNax3 映射到 1.3-cM 的间隔内。来自相应的水稻和短柄草间隔的基因编码 16 类不同的蛋白质，包括 HvNax3 的几个合理候选基因。讨论了 HvNax3 在提供有助于栽培大麦耐盐性的有用性状方面的潜力。
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