Graduate School of Life and Environmental Sciences, University of Tsukuba , Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572 , Japan.
Institute of Plant Science and Resources, Okayama University , Chuo 2-20-1, Kurashiki, Okayama 710-0046 , Japan.
Breed Sci. 2014 Dec;64(4):378-88. doi: 10.1270/jsbbs.64.378. Epub 2014 Dec 1.
Two hundred ninety-six Asian barley (Hordeum vulgare L.) accessions were assessed to detect QTLs underlying salt tolerance by association analysis using a 384 single nucleotide polymorphism (SNP) marker system. The experiment was laid out at the seedling stage in a hydroponic solution under control and 250 mM NaCl solution with three replications of four plants each. Salt tolerance was assessed by leaf injury score (LIS) and salt tolerance indices (STIs) of the number of leaves (NL), shoot length (SL), root length (RL), shoot dry weight (SDW) and root dry weight (RDW). LIS was scored from 1 to 5 according to the severity of necrosis and chlorosis observed on leaves. There was a wide variation in salt tolerance among Asian barley accessions. LIS and STI (SDW) were the most suitable traits for screening salt tolerance. Association was estimated between markers and traits to detect QTLs for LIS and STI (SDW). Seven significant QTLs were located on chromosomes 1H (2 QTLs), 2H (2 QTLs), 3H (1 QTL), 4H (1 QTL) and 5H (1 QTL). Five QTLs were associated with LIS and 2 QTLs with STI (SDW). Two QTLs associated with LIS were newly identified on chromosomes 3H and 4H.
二百九十六份亚洲大麦（Hordeum vulgare L.）材料通过使用 384 个单核苷酸多态性（SNP）标记系统的关联分析来评估耐盐性的 QTL。该实验在幼苗期在水培溶液中进行，在对照和 250mM NaCl 溶液下进行，每个处理有三个重复，每个重复有四株植物。通过叶片损伤评分（LIS）和耐盐指数（STI）评估耐盐性，耐盐指数包括叶片数（NL）、株高（SL）、根长（RL）、地上部干重（SDW）和根干重（RDW）。根据叶片上观察到的坏死和黄化程度，LIS 从 1 到 5 进行评分。亚洲大麦材料之间存在广泛的耐盐性变异。LIS 和 STI（SDW）是筛选耐盐性的最适宜性状。估计标记与性状之间的关联，以检测 LIS 和 STI（SDW）的 QTL。在 1H（2 个 QTL）、2H（2 个 QTL）、3H（1 个 QTL）、4H（1 个 QTL）和 5H（1 个 QTL）上定位到 7 个显著 QTL。5 个 QTL 与 LIS 相关，2 个 QTL 与 STI（SDW）相关。与 LIS 相关的两个 QTL 是在 3H 和 4H 染色体上新鉴定的。
