Cheng Hai, Cao Jiang, Chen Wei, Qi Kun-Ming, Yan Zhi-Ling, Xu Kai-Lin
Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Jun;25(3):843-846. doi: 10.7534/j.issn.1009-2137.2017.03.038.
To evaluate the clinical significance of thrombopoietin (TPO) level in diagnosis of pateints with aplastic anaemia (AA).
The TPO levels in sera from 54 AA patients and 119 healthy controls were examined. A total of 92 samples were collected from AA patients including 43 samples harvested at diagnosis, 23 samples in the cytopenic period after treatment, and 26 samples of patients in partial (n=10) or complete remission (n=16) following immunosuppressive treatment. Serum TPO levels were assessed by a sandwich-antibody ELISA that utilized a polyclonal rabbit antiserum for both capture and signal.
Serum samples from normal donors revealed a mean TPO level of 95.3±54.0 pg/ml, the mean TPO levels in AA sera collected at diagnosis and before onset of treatment were 2728±1074 pg/ml (P<0.001) compared with normal controls; mean platelet count at that time: 27×10/L). Serum TPO levels of AA patients in partial or complete remission after immunosuppressive treatment were significantly lower than TPO levels at diagnosis (P<0.001). However, despite normal platelet counts (mean 167×10/L), TPO levels remained significantly elevated in complete remission (mean TPO 1009±6590 pg/ml (P<0.001) compared with normal controls. There was a significant negative correlation between serum TPO levels and platelet counts in AA patients (coefficient of correlation r=-0.70, P<0.001).
TPO levels are highly elevated in sera of patients with AA. Thrombopoietin did not return to normal levels in remission, indicating normal platelet count in remission of AA needs sustained high level of TPO.
评估血小板生成素（TPO）水平在再生障碍性贫血（AA）患者诊断中的临床意义。
检测了54例AA患者和119例健康对照者血清中的TPO水平。从AA患者中总共采集了92份样本，其中包括诊断时采集的43份样本、治疗后血细胞减少期的23份样本以及免疫抑制治疗后部分缓解（n = 10）或完全缓解（n = 16）患者的26份样本。采用双抗体夹心ELISA法评估血清TPO水平，该方法使用多克隆兔抗血清进行捕获和信号检测。
正常供者血清样本显示TPO平均水平为95.3±54.0 pg/ml，AA患者诊断时及治疗前采集的血清中TPO平均水平为2728±1074 pg/ml（与正常对照相比，P<0.001）；此时平均血小板计数为27×10⁹/L）。免疫抑制治疗后部分或完全缓解的AA患者血清TPO水平显著低于诊断时的TPO水平（P<0.001）。然而，尽管血小板计数正常（平均167×10⁹/L），完全缓解时TPO水平仍显著升高（平均TPO为1009±6590 pg/ml，与正常对照相比，P<0.001）。AA患者血清TPO水平与血小板计数之间存在显著负相关（相关系数r = -0.70，P<0.001）。
AA患者血清中TPO水平显著升高。缓解期血小板生成素未恢复至正常水平，表明AA缓解期正常的血小板计数需要持续高水平的TPO。
