Halder Binata, Malakar Arup Kumar, Chakraborty Supriyo
Department of Biotechnology, Assam University, Silchar-788011, Assam, India.
Bioinformation. 2017 Feb 28;13(2):46-53. doi: 10.6026/97320630013046. eCollection 2017.
The basic sequence features were analysed that influence gene expression via codon usage bias of the selected forty coding sequences of Homo sapiens in a simple prokaryotic model i.e. E. coli K-12 genome. The prime objective was to elucidate the interrelationships among tRNA gene copy numbers, synonymous codons, amino acids and translational efficiency using tRNA adaptation index. It was evident from RSCU scores and principal component analysis, that only those preferred codons were used by the isoacceptor tRNAs that had G and C base at their third codon position. Relationship between tRNA adaptation index and amino acids, revealed that valine, arginine, serine and isoleucine showed significant positive correlation with gene expression. Therefore, it could be inferred that GC content in these genes might have the major role in shaping the codon bias and affecting the translational efficiency of the coding sequences.
在一个简单的原核模型即大肠杆菌K-12基因组中，分析了通过所选40个人类编码序列的密码子使用偏好影响基因表达的基本序列特征。主要目的是使用tRNA适应指数阐明tRNA基因拷贝数、同义密码子、氨基酸和翻译效率之间的相互关系。从相对同义密码子使用（RSCU）得分和主成分分析可以明显看出，只有那些在其第三个密码子位置具有G和C碱基的同功受体tRNA使用偏好密码子。tRNA适应指数与氨基酸之间的关系表明，缬氨酸、精氨酸、丝氨酸和异亮氨酸与基因表达呈显著正相关。因此，可以推断这些基因中的GC含量可能在塑造密码子偏好和影响编码序列的翻译效率方面起主要作用。
