Ederli Luisa, Brunetti Cecilia, Centritto Mauro, Colazza Stefano, Frati Francesca, Loreto Francesco, Marino Giovanni, Salerno Gianandrea, Pasqualini Stefania
Department of Chemistry, Biology and Biotechnology, University of PerugiaPerugia, Italy.
Trees and Timber Institute, National Research Council of ItalySesto Fiorentino, Italy.
Front Plant Sci. 2017 Jun 8;8:959. doi: 10.3389/fpls.2017.00959. eCollection 2017.
The response of broad bean () plants to water stress alone and in combination with green stink bug () infestation was investigated through measurement of: (1) leaf gas exchange; (2) plant hormone titres of abscisic acid (ABA) and its metabolites, and of salicylic acid (SA); and (3) hydrogen peroxide (HO) content. Furthermore, we evaluated the effects of experimentally water-stressed broad-bean plants on performance in terms of adult host-plant preference, and nymph growth and survival. Water stress significantly reduced both photosynthesis () and stomatal conductance ( ), while infestation by the green stink bug had no effects on photosynthesis but significantly altered partitioning of ABA between roots and shoots. Leaf ABA was decreased and root ABA increased as a result of herbivore attack, under both well-watered and water-deprived conditions. Water stress significantly impacted on SA content in leaves, but not on HO. However, infestation of greatly increased both SA and HO contents in leaves and roots, which suggests that endogenous SA and HO have roles in plant responses to herbivore infestation. No significant differences were seen for green stink bug choice between well-watered and water-stressed plants. However, for green stink bug nymphs, plant water stress promoted significantly lower weight increases and significantly higher mortality, which indicates that highly water-stressed host plants are less suitable for infestation. In conclusion two important findings emerged: (i) association of water stress with herbivore infestation largely changes plant response in terms of phytohormone contents; but (ii) water stress does not affect the preference of the infesting insects, although their performance was impaired.
通过测量以下指标，研究了蚕豆（ ）植株对单独水分胁迫以及水分胁迫与绿蝽（ ）侵害相结合情况的响应：（1）叶片气体交换；（2）脱落酸（ABA）及其代谢产物以及水杨酸（SA）的植物激素含量；（3）过氧化氢（HO）含量。此外，我们从成虫对寄主植物的偏好、若虫生长和存活方面评估了实验性水分胁迫蚕豆植株对 表现的影响。水分胁迫显著降低了光合作用（ ）和气孔导度（ ），而绿蝽侵害对光合作用没有影响，但显著改变了ABA在根和地上部之间的分配。在水分充足和缺水条件下，食草动物攻击均导致叶片ABA含量降低，根中ABA含量增加。水分胁迫显著影响叶片中SA含量，但对HO无影响。然而， 的侵害显著增加了叶片和根中SA和HO的含量，这表明内源性SA和HO在植物对食草动物侵害的反应中起作用。在水分充足和水分胁迫的植株之间，绿蝽的选择没有显著差异。然而，对于绿蝽若虫来说，植物水分胁迫显著促进了较低的体重增加和较高的死亡率，这表明高度水分胁迫的寄主植物不太适合 侵害。总之，出现了两个重要发现：（i）水分胁迫与食草动物侵害的关联在很大程度上改变了植物在植物激素含量方面的反应；但（ii）水分胁迫不影响侵害昆虫的偏好性，尽管它们的表现受到了损害。
