Sabljic Ivana, Barneto Jesica A, Balestrasse Karina B, Zavala Jorge A, Pagano Eduardo A
Instituto de Investigaciones en Biociencias Agrícolas y Ambientales-INBA, Facultad de Agronomía, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.
GDM, Chacabuco, Buenos Aires, Argentina.
PeerJ. 2020 Sep 17;8:e9956. doi: 10.7717/peerj.9956. eCollection 2020.
Southern green stink bugs ( L.) are one of the major pests in many soybean producing areas. They cause a decrease in yield and affect seed quality by reducing viability and vigor. Alterations have been reported in the oxidative response and in the secondary metabolites in different plant species due to insect damage. However, there is little information available on soybean-stink bug interactions. In this study we compare the response of undamaged and damaged seeds by in two soybean cultivars, IAC-100 (resistant) and Davis (susceptible), grown under greenhouse conditions. Pod hardness, HO generation, enzyme activities in guaiacol peroxidase (GPOX), catalase (CAT) and superoxide dismutase (SOD) as well as lipoxygenase expression and isoflavonoid production were quantified. Our results showed a greater resistance of IAC-100 to pod penetration, a decrease in peroxide content after stink bug attack, and higher GPOX, CAT and SOD activities in seeds due to the genotype and to the genotype-interaction with the herbivory treatment. Induction of expression in both cultivars and higher production of isoflavonoids in IAC-100 were also detected. It was then concluded that the herbivory stink bug induces pathways related to oxidative stress and to the secondary metabolites in developing seeds of soybean and that differences between cultivars hold promise for a plant breeding program.
南方绿蝽是许多大豆产区的主要害虫之一。它们会导致产量下降，并通过降低种子活力和生命力影响种子质量。据报道，不同植物物种因昆虫损害，其氧化反应和次生代谢产物会发生改变。然而，关于大豆与绿蝽相互作用的信息却很少。在本研究中，我们比较了在温室条件下种植的两个大豆品种IAC - 100（抗性品种）和戴维斯（敏感品种）中，未受损种子和受绿蝽损害种子的反应。对豆荚硬度、过氧化氢生成量、愈创木酚过氧化物酶（GPOX）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的酶活性以及脂氧合酶表达和异黄酮生成进行了量化。我们的结果表明，IAC - 100对豆荚穿透具有更强的抗性，绿蝽攻击后过氧化物含量降低，并且由于基因型以及基因型与食草处理的相互作用，种子中的GPOX、CAT和SOD活性更高。在两个品种中均检测到脂氧合酶表达上调，并且IAC - 100中异黄酮生成量更高。由此得出结论，绿蝽取食会诱导大豆发育种子中与氧化应激和次生代谢产物相关的途径，并且品种间的差异为植物育种计划带来了希望。
