School of Chemical and Chemistry Engineering, Yancheng Institute of Technology, Yancheng, China.
College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
Bioprocess Biosyst Eng. 2017 Sep;40(9):1427-1436. doi: 10.1007/s00449-017-1800-4. Epub 2017 Jun 22.
In this study, sequential biological pretreatment (BP) with Galactomyces sp. CCZU11-1 at 30 °C for 3 days and deep eutectic solvent (DES) choline chloride: oxalic acid (ChCl:OA, 1 mol/2 mol) extraction at 120 °C for 1.5 h was used for pretreating BSS. It was found that combination pretreatment could effectively remove xylan and lignin for enhancing enzymatic saccharification. The reducing sugars and glucose from the hydrolysis of 100 g/L pretreated BSS with complexed cellulases of Galactomyces sp. CCZU11-1 were obtained in the yields of 81.0% and 74.1%, respectively. The BSS-hydrolyzates had no inhibitory effects on the lipid-accumulating microorganism Bacillus sp. CCZU11-1, and the cell mass and TAG accumulation were 4.8 g CDW/L and 2.2 g TAG/L, respectively. Fatty acids including palmitic acid (C16:0; 25.3%), palmitoleic acid (C16:1; 24.4%), stearic acid (C18:0; 15.1%), and oleic acid (C18:1; 21.6%) were accumulated in cells. Clearly, this combination pretreatment has high potential application in future.
在这项研究中，采用酿酒酵母 CCZU11-1 进行连续生物预处理（BP），在 30°C 下处理 3 天，然后用深共熔溶剂（DES）氯化胆碱：草酸（ChCl:OA，1mol/2mol）在 120°C 下提取 1.5 小时，用于预处理 BSS。结果发现，组合预处理可以有效地去除木聚糖和木质素，从而增强酶解糖化作用。用酿酒酵母 CCZU11-1 的复合纤维素酶水解 100g/L 预处理 BSS，可得到 81.0%和 74.1%的还原糖和葡萄糖产率。BSS 水解产物对产油微生物芽孢杆菌 CCZU11-1 没有抑制作用，细胞干重和 TAG 积累分别为 4.8g CDW/L 和 2.2g TAG/L。细胞中积累了多种脂肪酸，包括棕榈酸（C16:0；25.3%）、棕榈油酸（C16:1；24.4%）、硬脂酸（C18:0；15.1%）和油酸（C18:1；21.6%）。显然，这种组合预处理在未来具有很高的潜在应用价值。
