通过碱提取与深共晶溶剂浸泡联合预处理实现高粱秸秆的有效脱木质素，从而增强酶解糖化。
Enhanced enzymatic saccharification of sorghum straw by effective delignification via combined pretreatment with alkali extraction and deep eutectic solvent soaking.
机构信息
National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, School of Pharmacy, Changzhou University, Changzhou, Jiangsu Province 213164, PR China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei Province 430062, PR China.
出版信息
Bioresour Technol. 2021 Nov;340:125695. doi: 10.1016/j.biortech.2021.125695. Epub 2021 Jul 31.
Hydrogen bond donor (HBD) in ChCl-based deep eutectic solvent (DESs) had significant influence on the Sorghum straw (SS) pretreatment. Lactic acid (LAC) was chosen as the appropriate HBD for preparing ChCl-based DES to pretreat Sorghum straw (SS). Furthermore, sequential pretreatment with dilute sodium hydroxide (0.75 wt%) for 1 h at 121 °C and ChCl:LAC soaking at 140 °C for 40 min was applied to pretreat SS for removing lignin (78.4%) and xylan (67.6%). Hydrolysis for 72 h, the reducing sugar yield reached 94.9%. Moreover, relationships of delignification and xylan removal with saccharification were explored after pretreatment. Finally, the fermentability of SS-hydrolysates was verified by bioethanol fermentation by S. cerevissiae with the yield of 0.45 g ethanol/g glucose. No significant inhibition was observed on ethanol fermentation. Obviously, establishment of high-efficient combination pretreatment with alkali extraction and ChCl:LAC soaking was successfully demonstrated for enhancing enzymatic saccharification of SS.
在基于氯化胆碱的深共熔溶剂（DESs）中，氢键供体（HBD）对高粱秸秆（SS）预处理有重要影响。选择乳酸（LAC）作为合适的 HBD 来制备基于氯化胆碱的 DES 以预处理高粱秸秆（SS）。此外，采用 0.75wt%稀氢氧化钠在 121°C 下预处理 1 小时，然后在 140°C 下用氯化胆碱：LAC 浸泡 40 分钟，用于去除木质素（78.4%）和木聚糖（67.6%）。水解 72 小时后，还原糖产率达到 94.9%。此外，在预处理后还探讨了脱木质素和木聚糖去除与糖化的关系。最后，通过酿酒酵母的生物乙醇发酵验证了 SS 水解物的发酵性，葡萄糖得率为 0.45g 乙醇/g 葡萄糖。乙醇发酵过程中没有观察到明显的抑制作用。显然，成功证明了采用碱提取和氯化胆碱：LAC 浸泡的高效组合预处理方法可以提高 SS 的酶糖化效率。
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