猴子的基因组编辑

Genome Editing of Monkey.

作者信息

Liu Zhen, Cai Yijun, Sun Qiang

机构信息

Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences (CAS) Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, CAS, Shanghai, 200031, China.

Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences, Room A2304, Biological Research Building, 320 Yue Yang Road, Shanghai, 200031, China.

出版信息

Methods Mol Biol. 2017;1630:141-152. doi: 10.1007/978-1-4939-7128-2_12.

DOI:10.1007/978-1-4939-7128-2_12

PMID:28643256

Abstract

Gene-modified monkey models would be particularly valuable in biomedical and neuroscience research. Virus-based transgenic and programmable nucleases-based site-specific gene editing methods (TALEN, CRISPR-cas9) enable the generation of gene-modified monkeys with gain or loss of function of specific genes. Here, we describe the generation of transgenic and knock-out (KO) monkeys with high efficiency by lentivirus and programmable nucleases.

摘要

基因修饰猴模型在生物医学和神经科学研究中具有特别重要的价值。基于病毒的转基因方法以及基于可编程核酸酶的位点特异性基因编辑方法（转录激活样效应因子核酸酶、规律成簇间隔短回文重复序列及其相关蛋白9）能够培育出特定基因功能获得或缺失的基因修饰猴。在此，我们描述了通过慢病毒和可编程核酸酶高效培育转基因猴和基因敲除猴的方法。