利用转录激活样效应因子核酸酶对海鞘肠鳃纲动物进行基因组编辑。

Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.

作者信息

Sasakura Yasunori, Yoshida Keita, Treen Nicholas

机构信息

Shimoda Marine Research Center, University of Tsukuba, 5-10-1, Shimoda, Shizuoka, 415-0025, Japan.

出版信息

Methods Mol Biol. 2017;1630:235-245. doi: 10.1007/978-1-4939-7128-2_19.

DOI:10.1007/978-1-4939-7128-2_19

PMID:28643263

Abstract

The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.

摘要

海鞘（Ciona intestinalis）是研究构建脊索动物身体发育机制的重要模式动物。尽管很早以前就开发出了用于操作海鞘基因的分子和胚胎学技术，但该动物在基因组编辑方面的最新成果革新了对海鞘基因的功能分析。特别是，在G0代敲除基因并结合TALENs的组织特异性表达，使我们能够快速研究以往方法难以解决的基因功能。