Old D C, Tavendale A, Senior B W
J Med Microbiol. 1985 Oct;20(2):203-14. doi: 10.1099/00222615-20-2-203.
Type-3 fimbriae isolated from members of five different species of Klebsiella were 4-5 nm in diameter and agglutinated the tannic acid-treated erythrocytes of ox and, in some cases, the untanned erythrocytes of fowl. In sodium dodecyl sulphate-polyacrylamide gel electrophoresis, the type-3 fimbrial proteins had mol. wts in the range 19 500-21 500. Hydrophobic amino acids comprised 39.6% of all the amino acids of the type-3 fimbrial protein of K. oxytoca strain 70/1. The type-1 fimbrial protein of Klebsiella had a mol. wt of c. 18 000 and the type-1 fimbriae were serologically distinct from the type-3 fimbriae. Our results for the type-3 fimbriae of Klebsiella were compared with those of others for morphologically similar and serologically related thin fimbriae of Salmonella and Yersinia.
从五种不同克雷伯氏菌属细菌中分离出的3型菌毛直径为4 - 5纳米,能凝集经单宁酸处理的牛红细胞,在某些情况下还能凝集未鞣制的禽红细胞。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中,3型菌毛蛋白的分子量在19500 - 21500范围内。产酸克雷伯氏菌70/1菌株的3型菌毛蛋白中,疏水氨基酸占所有氨基酸的39.6%。克雷伯氏菌的1型菌毛蛋白分子量约为18000,且1型菌毛与3型菌毛在血清学上不同。我们对克雷伯氏菌3型菌毛的研究结果与其他人对形态相似且血清学相关的沙门氏菌和耶尔森氏菌细菌毛的研究结果进行了比较。