Lv Duo, Zhou Dan, Zhang Yan, Zhang Shuai, Zhu Yi-Min
Department of Epidemiology & Biostatistics, Zhejiang University School of Public Health, Hangzhou 310058, China; Research Center of Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, Zhejiang University, Hangzhou 310058, China.
Department of Epidemiology & Biostatistics, Zhejiang University School of Public Health, Hangzhou 310058, China.
Gene. 2017 Sep 5;627:284-289. doi: 10.1016/j.gene.2017.06.030. Epub 2017 Jun 20.
Genome-wide association studies have identified novel obesity-associated susceptibility loci. Associations of these variants with childhood obesity have been studied in our previous research. The purpose of this study is to investigate if these loci are associated with hypertension being independent of obesity in Chinese children and adolescents.
Nineteen candidate SNPs were genotyped using Sequenom MassARRAY platform among Chinese children (N=2954, 514 hypertension and 2440 controls, aged 7-17years). Dietary behaviors were assessed through face to face investigations.
Of the nineteen obese related SNPs, ten SNPs were found to be associated with systolic blood pressure (SBP) or diastolic blood pressure (DBP) in Chinese children. After adjusting for age, sex and WHtR, rs2605100 in LYPLAL1was found to be associated with high blood pressure (HBP) under dominant model (P=0.024) with the OR of 1.274 (95% CI =1.033-1.572, effect genotype=GG). The distribution of genotype of rs7647305 in ETV5 showed significant difference between HBP and non-HBP subjects under dominant model (P=0.011) with the OR of 0.654 (95% CI=0.471-0.909, effect genotype=CC). Using rs2605100 and rs7647305, the genetic risk score (GRS) analysis showed that, after adjusted for age, sex and WHtR, subjects carrying one or two risk alleles had the risks of hypertension with the ORs 1.797 (95% CI, 1.168-2.765), 2.149 (95% CI, 1.375-3.357) comparing with the subjects with non-risk-allele.
Genetic variations of obesity-associated loci, LYPLAL1 rs2605100 and ETV5 rs7647305 independently associate with the risk of childhood hypertension in China.
全基因组关联研究已经确定了新的肥胖相关易感基因座。在我们之前的研究中已经对这些变异与儿童肥胖的关联进行了研究。本研究的目的是调查在中国儿童和青少年中，这些基因座是否独立于肥胖与高血压相关。
使用Sequenom MassARRAY平台对2954名中国儿童（514名高血压患者和2440名对照，年龄7至17岁）进行19个候选单核苷酸多态性（SNP）基因分型。通过面对面调查评估饮食行为。
在这19个与肥胖相关的SNP中，发现有10个SNP与中国儿童的收缩压（SBP）或舒张压（DBP）相关。在调整年龄、性别和腰高比（WHtR）后，发现LYPLAL1基因中的rs2605100在显性模型下与高血压（HBP）相关（P = 0.024），比值比（OR）为1.274（95%可信区间[CI]=1.033 - 1.572，效应基因型=GG）。在显性模型下，ETV5基因中rs7647305的基因型分布在高血压患者和非高血压患者之间存在显著差异（P = 0.011），OR为0.654（95%CI = 0.471 - 0.909，效应基因型=CC）。使用rs2605100和rs7647305进行遗传风险评分（GRS）分析表明，在调整年龄、性别和WHtR后，携带一个或两个风险等位基因的受试者患高血压的风险与非风险等位基因受试者相比，OR分别为1.797（95%CI，1.168 - 2.765）、2.149（95%CI，1.375 - 3.357）。
肥胖相关基因座LYPLAL1的rs2605100和ETV5的rs7647305的基因变异独立与中国儿童期高血压风险相关。
