Kay Vanessa R, Tayade Chandrakant, Carmeliet Peter, Croy B Anne
Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
Laboratory of Angiogenesis and Vascular Metabolism, VIB - Vesalius Research Center, University of Leuven, Department of Oncology, Leuven, Belgium.
Dev Dyn. 2017 Sep;246(9):700-712. doi: 10.1002/dvdy.24540. Epub 2017 Jul 24.
Placental growth factor (PGF) is important for wound-healing and vascular collaterogenesis. PGF deficiency is associated with preeclampsia, a hypertensive disease of human pregnancy. Offspring born to preeclamptic mothers display cognitive impairments and brain vascular and neurostructural deviations. Low PGF production during development may contribute to alterations in offspring cerebrovascular beds. Retina is a readily accessible part of the central nervous system with a well-described pattern of vascular development in mice. Impacts of PGF deficiency were addressed during mouse retinal vascularization.
Retinal vessels were compared between Pgf and congenic C57BL/6 (B6) mice. PGF deficiency altered neonatal retinal vascularization patterns. Some anatomic alterations persisted into adulthood, particularly in males. Greater arterial wall collagen IV expression was found in adult Pgf females. Pregnancy (studied in adult females at gestational days 11.5 or 18.5) induced subtle changes upon the mother's retinal vasculature but these pregnancy-induced changes did not differ between genotypes. Significant sex-related differences occurred between adult male and female B6 although sexually dimorphic retinal vascular differences were absent in B6 neonates.
Overall, PGF has a role in retinal vascular angiogenesis and vessel organization during development but does not affect retinal vessel adaptations in adult females during pregnancy. Developmental Dynamics 246:700-712, 2017. © 2017 Wiley Periodicals, Inc.
胎盘生长因子（PGF）对伤口愈合和血管侧支生成很重要。PGF缺乏与子痫前期有关，子痫前期是人类妊娠期的一种高血压疾病。子痫前期母亲所生的后代表现出认知障碍以及脑血管和神经结构异常。发育过程中PGF产生不足可能导致后代脑血管床的改变。视网膜是中枢神经系统易于观察的部分，其在小鼠中的血管发育模式已被充分描述。本研究探讨了PGF缺乏对小鼠视网膜血管生成的影响。
比较了Pgf基因敲除小鼠和同基因C57BL/6（B6）小鼠的视网膜血管。PGF缺乏改变了新生小鼠视网膜血管生成模式。一些解剖学改变持续到成年期，尤其是在雄性小鼠中。在成年Pgf基因敲除雌性小鼠中发现动脉壁胶原蛋白IV表达增加。妊娠（在成年雌性小鼠妊娠第11.5天或18.5天进行研究）对母亲的视网膜血管系统产生了细微变化，但这些妊娠诱导的变化在不同基因型之间没有差异。成年雄性和雌性B6小鼠之间存在显著的性别相关差异，尽管B6新生小鼠不存在性别差异的视网膜血管差异。
总体而言，PGF在发育过程中对视网膜血管生成和血管组织有作用，但在成年雌性小鼠妊娠期间不影响视网膜血管的适应性变化。《发育动力学》2017年第246卷：700 - 712页。© 2017威利期刊公司。
