CRISPR 相关核酸酶的构象调控。
Conformational regulation of CRISPR-associated nucleases.
机构信息
Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, United States.
Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, United States.
出版信息
Curr Opin Microbiol. 2017 Jun;37:110-119. doi: 10.1016/j.mib.2017.05.010. Epub 2017 Jun 21.
Abstract
Adaptive immune systems in bacteria and archaea rely on small CRISPR-derived RNAs (crRNAs) to guide specialized nucleases to foreign nucleic acids. The activation of these nucleases is controlled by a series of molecular checkpoints that ensure precise cleavage of nucleic acid targets, while minimizing toxic off-target cleavage events. In this review, we highlight recent advances in understanding regulatory mechanisms responsible for controlling the activation of these nucleases and identify emerging regulatory themes conserved across diverse CRISPR systems.
摘要
细菌和古菌中的适应性免疫系统依赖于小型 CRISPR 衍生的 RNA（crRNA）来引导专门的核酸酶靶向外来核酸。这些核酸酶的激活受到一系列分子检测点的控制，以确保核酸靶标精确切割，同时最大限度减少毒性非靶标切割事件。在这篇综述中，我们强调了最近在理解控制这些核酸酶激活的调节机制方面的进展，并确定了在不同的 CRISPR 系统中保守的新兴调节主题。
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