Comby Morgane, Gacoin Marie, Robineau Mathilde, Rabenoelina Fanja, Ptas Sébastien, Dupont Joëlle, Profizi Camille, Baillieul Fabienne
Institut de Systématique, Evolution et Biodiversité, ISYEB - UMR 7205 - CNRS, MNHN, UPMC, EPHE, Muséum national d'histoire naturelle, Sorbonne Universités, 57 rue Cuvier, CP39, 75005 Paris, France; Soufflet Biotechnologies, Quai Sarrail, 10400 Nogent-sur-Seine, France; Laboratoire de Stress, Défenses et Reproduction des Plantes - EA 4707, Faculté des Sciences, Moulin de la Housse, BP 1039, 51687 Reims cedex 2, France.
Soufflet Biotechnologies, Quai Sarrail, 10400 Nogent-sur-Seine, France.
Microbiol Res. 2017 Sep;202:11-20. doi: 10.1016/j.micres.2017.04.014. Epub 2017 May 8.
In order to find biological control agents (BCAs) for the management of Fusarium head blight (FHB), a major disease on wheat crops worldwide, 86 microorganisms isolated from inner tissues of wheat plants were discriminated for their ability to inhibit the growth of Fusarium graminearum and Fusarium culmorum by in vitro dual culture assays. A group of 22 strains appeared very effective to inhibit F. graminearum (inhibition of 30-51%) and they were also globally effective in controlling F. culmorum (inhibition of 15-53%). Further evaluation of a subselection of strains by screening on detached spikelets in vitro confirmed three species, namely Phoma glomerata, Aureobasidium proteae and Sarocladium kiliense, that have not yet been reported for their efficacy against Fusarium spp., indicating that looking for BCAs toward FHB among wheat endophytes proved to be promising. The efficacy of some strains turned out different between both in vitro screening approaches, raising the importance of finding the most appropriate screening approach for the search of BCAs. This study pointed out the interest of the test on detached wheat spikelets that provided information about a potential pathogenicity, the growth capacity and efficacy of the endophyte strains on the targeted plant, before testing them on whole plants.
为了寻找用于防治小麦赤霉病(FHB)的生防菌,小麦赤霉病是全球小麦作物上的一种主要病害,通过体外对峙培养试验,对从小麦植株内部组织分离出的86种微生物抑制禾谷镰刀菌和燕麦镰刀菌生长的能力进行了鉴别。一组22个菌株对抑制禾谷镰刀菌表现出非常有效的作用(抑制率为30%-51%),并且它们在总体上对控制燕麦镰刀菌也有效(抑制率为15%-53%)。通过在离体小穗上进行体外筛选对菌株的一个子集进行进一步评估,确认了3个物种,即聚生茎点霉、普罗蒂奥氏 Aureobasidium proteae 和基利安帚枝霉,它们对镰刀菌属的防治效果尚未见报道,这表明在小麦内生菌中寻找防治小麦赤霉病的生防菌被证明是有前景的。在两种体外筛选方法中,一些菌株的效果有所不同,这凸显了找到最适合筛选生防菌的方法的重要性。这项研究指出了对离体小麦小穗进行测试的意义,即在对全株进行测试之前,该测试能提供有关内生菌菌株对目标植物的潜在致病性、生长能力和防治效果的信息。