Xu Yi-Gang, Chai Long-Hui, Shi Wen, Wang Dan-Dan, Zhang Jing-Yu, Xiao Xiang-Hong
College of Wildlife Resources, Northeast Forestry University, Harbin, China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Appl Microbiol Biotechnol. 2017 Jul;101(14):5799-5808. doi: 10.1007/s00253-017-8385-3. Epub 2017 Jun 24.
Recently, populations of Rana dybowskii, an important amphibian species in Northeast China, have decreased, mainly owing to the disease caused by Aeromonas hydrophila. However, effective control methods have not yet been developed. In order to explore the immune responses of R. dybowskii upon exposure to A. hydrophila infection, Illumina high-throughput transcriptome sequencing and digital gene expression (DGE) technology were employed to investigate transcriptomic changes in the skin of R. dybowskii exposed to A. hydrophila. In this work, a total of 26,244,446 transcriptome sequencing reads were obtained and assembled into 109,089 unique unigenes using de novo assembly, and a total of 37,105 unigenes (34.0%) were functionally annotated against the non-redundant (Nr), Swiss-Prot, Cluster of Orthologous Groups of Proteins (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Ontology (GO) databases. Gene expression changes in the skin tissue of R. dybowskii exposed to A. hydrophila were investigated by a tag-based DGE system, and a total of 1435 significantly differentially expressed genes were identified, including 460 that were up-regulated and 975 that were down-regulated, indicating a large change in the host transcriptome profile exposed to A. hydrophila. Among these, 478 genes were associated with immune-relevant pathways, metabolic pathways, cellular components, growth, migration, and muscle and hormone signaling pathways. We confirmed the differential expression of 106 immune-relevant genes associated with innate and adaptive immune responses. Our data provide a fairly comprehensive molecular biology background for the deeper understanding of the amphibian immune system following A. hydrophila infection.
最近，东北重要两栖物种东北林蛙的种群数量有所减少，主要是由嗜水气单胞菌引起的疾病所致。然而，尚未开发出有效的控制方法。为了探究东北林蛙暴露于嗜水气单胞菌感染后的免疫反应，采用了Illumina高通量转录组测序和数字基因表达（DGE）技术来研究暴露于嗜水气单胞菌的东北林蛙皮肤中的转录组变化。在这项工作中，共获得了26,244,446条转录组测序读数，并使用从头组装将其组装成109,089个独特的单基因，共有37,105个单基因（34.0%）根据非冗余（Nr）、瑞士蛋白质数据库（Swiss-Prot）、直系同源蛋白簇（COG）、京都基因与基因组百科全书（KEGG）和基因本体论（GO）数据库进行了功能注释。通过基于标签的DGE系统研究了暴露于嗜水气单胞菌的东北林蛙皮肤组织中的基因表达变化，共鉴定出1435个显著差异表达基因，其中460个上调，975个下调，这表明暴露于嗜水气单胞菌的宿主转录组谱发生了很大变化。其中，478个基因与免疫相关途径、代谢途径、细胞成分、生长、迁移以及肌肉和激素信号通路有关。我们证实了106个与先天和适应性免疫反应相关的免疫相关基因的差异表达。我们的数据为更深入了解嗜水气单胞菌感染后两栖动物的免疫系统提供了相当全面的分子生物学背景。
