Lei H Q, Cheng M L, Li J, Zhou P P, Luo X H, Yu F
Department of Infectious Diseases, Guizhou Medical University, Guizhou 550004, China.
Zhonghua Yi Xue Za Zhi. 2017 Jun 20;97(23):1815-1819. doi: 10.3760/cma.j.issn.0376-2491.2017.23.012.
To evaluate the effect of human placental extracts (HPE) on the protection from acute liver injury (ALI) induced by D-GalN and analyze the components of HPE. (1)Fourty male mice were randomly divided into five groups (Blank, Model, MgIG, HPE and HPE+ MgIG) for the ALI model and treatments.The serum alanine aminotransferase (ALT) and aspartate transaminase (AST) were determined by biochemical assays.Nitric monoxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) in serum and hepatic tissue were detected by assay kits.The extent of liver damage was evaluated by histological examination.(2)Relative molecular mass of HPE was determined by SDS-PAGE.(3) Component identification was performed by using LCMS-TOF.(4)Selected functional molecules in HPE were detected by protein array. (1) A lower level of NO and MDA and a higher SOD and T-AOC were observed in rats treated with HPE compared to the non-treated rats in an acute liver failure disease model.(2) The size of HPE was about 1 200-4 600 by electrophores.(3) 7 peaks of HPE were identified, including uracil, hypoxanthine, tyrosine, phenylalanine, tryptophan, xanthine and thymine.(4) Comparable high concentrations of TGF-β, IGF-1, IL-9, IL-29 and TNF-α of HPE were revealed by protein array. (1) HPE protects rat from liver damage induced by D-GalN. (2) HPE contains Uracil, hypoxanthine, xanthine, thymine, and functional proteins as TGF-β, IGF-1, IL-9, IL-29 and TNF-α.
评估人胎盘提取物（HPE）对D-氨基半乳糖（D-GalN）诱导的急性肝损伤（ALI）的保护作用，并分析HPE的成分。（1）将40只雄性小鼠随机分为五组（空白组、模型组、甘草酸镁组、HPE组和HPE+甘草酸镁组）用于ALI模型和处理。通过生化检测测定血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）。使用试剂盒检测血清和肝组织中的一氧化氮（NO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）。通过组织学检查评估肝损伤程度。（2）通过SDS-PAGE测定HPE的相对分子质量。（3）使用LCMS-TOF进行成分鉴定。（4）通过蛋白质芯片检测HPE中选定的功能分子。（1）在急性肝衰竭疾病模型中，与未处理的大鼠相比，用HPE处理的大鼠中NO和MDA水平较低，SOD和T-AOC较高。（2）通过电泳法测定HPE的大小约为1200-4600。（3）鉴定出HPE的7个峰，包括尿嘧啶、次黄嘌呤、酪氨酸、苯丙氨酸、色氨酸、黄嘌呤和胸腺嘧啶。（4）蛋白质芯片显示HPE中TGF-β、IGF-1、IL-9、IL-29和TNF-α的浓度相当高。（1）HPE可保护大鼠免受D-GalN诱导的肝损伤。（2）HPE含有尿嘧啶、次黄嘌呤、黄嘌呤、胸腺嘧啶以及TGF-β、IGF-1、IL-9、IL-29和TNF-α等功能蛋白。
