Wu Tongqian, He Jingjing, Yan Shirong, Li Jing, Chen Ke, Zhang Dingshan, Cheng Mingliang, Xiang Zou, Fang Yu
Center for Clinical Laboratories, Affiliated Hospital of Guizhou Medical University, Guiyi Street 28, Guiyang, 550004, Guizhou, China.
School of Laboratory Science, Guizhou Medical University, Guiyang, China.
Allergy Asthma Clin Immunol. 2023 Nov 27;19(1):98. doi: 10.1186/s13223-023-00850-y.
Human placental extract (HPE) has been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported.
To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 or HMC-1 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model.
HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE.
This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells.
有文献记载，人胎盘提取物（HPE）有助于某些疾病（包括过敏）的愈合。然而，尚未有关于HPE对肥大细胞（过敏性疾病中的关键细胞类型）功能影响的报道。
为了研究HPE对肥大细胞生物学功能在过敏调节方面的影响，用HPE处理肥大细胞系C57或HMC-1细胞，然后评估细胞增殖、凋亡、活化、趋化性和吞噬作用。还研究了小鼠腹腔肥大细胞在体内对HPE诱导凋亡的反应。此外，使用被动皮肤过敏反应（PCA）试验（一种急性过敏模型）证实了HPE对肥大细胞脱颗粒的影响。
HPE能够抑制肥大细胞增殖并诱导肥大细胞凋亡。对化合物48/80或抗DNP IgE和DNP介导的活化的肥大细胞脱颗粒受到抑制。此外，用HPE处理会损害肥大细胞产生细胞因子和细胞趋化性。这些观察结果与用HPE处理后被动皮肤过敏反应（PCA）试验减弱一致。
本研究揭示了HPE对整体肥大细胞活性的抑制作用，表明HPE可能通过肥大细胞对减轻过敏性疾病具有调节作用。
