在三羟甲基氨基甲烷改性多孔玻璃中纯化蜱传脑炎病毒。
Purification of tick-borne encephalitis virus in tris-modified porous glasses.
作者信息
Krasilnikov I V, Elbert L B, Borisova V N, Nakhapetyan L A
出版信息
Acta Virol. 1985 Jul;29(4):273-8.
Macroporous glasses, chemically modified by tris-hydroxymethyl-aminomethane, were used for gel-permeation chromatography of tick-borne encephalitis (TBE) virus suspension. The highest yield of purified virus was observed in the carriers containing not less than 0.01 micrograms-equivalent/m2 surface-attached tris-hydroxymethyl-aminomethane groups at pH of the eluent higher than 7.6. The obtained modified porous carriers appeared to be suitable for the purification of both infectious and inactivated preparations of TBE virus.
用三羟甲基氨基甲烷进行化学修饰的大孔玻璃用于蜱传脑炎（TBE）病毒悬液的凝胶渗透色谱分析。在洗脱液pH高于7.6时，在每平方米表面附着不少于0.01微克当量三羟甲基氨基甲烷基团的载体中观察到纯化病毒的最高产量。所获得的改性多孔载体似乎适用于纯化TBE病毒的感染性制剂和灭活制剂。
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