Malenko G V, Fokina G I, Levina L S, Mamonenko L L, Rzhakhova O E, Pogodina V V, Frolova M P
Acta Virol. 1982 Sep;26(5):362-8.
Tick-borne encephalitis (TBE) virus was isolated from the brains and spinal cords, blood, livers, lymph nodes and kidneys from Macaca rhesus monkeys showing acute and subacute fatal encephalitis. In subacute encephalitis, virus titres in the CNS were lower than in acute disease (3.0--6.2 against 3.8--8.3 log LD50/ml). TBE virus localization in chronic encephalitis was largely the same as in acute and subacute disease. In monkeys with a chronic course and stable paralysis of the upper extremity, infectious TBE virus was isolated on day 383 from subcortical ganglia and spinal cord. In lymph nodes and spleen, it could be detected only by a combination of methods (co-cultivation in association with fluorescent antibody technique and complement-fixation test, explantation of organ fragments) more sensitive than is the inoculation of mice with organ homogenates. TBE virus was detected by the same methods on day 90 in the CNS and internal organs of a monkey with chronic encephalitis in the stage of remission.
从患有急性和亚急性致命性脑炎的恒河猴的脑、脊髓、血液、肝脏、淋巴结和肾脏中分离出蜱传脑炎（TBE）病毒。在亚急性脑炎中，中枢神经系统中的病毒滴度低于急性疾病（3.0--6.2对3.8--8.3 log LD50/ml）。TBE病毒在慢性脑炎中的定位与急性和亚急性疾病基本相同。在病程为慢性且上肢稳定麻痹的猴子中，在第383天从皮质下神经节和脊髓中分离出传染性TBE病毒。在淋巴结和脾脏中，只有通过比用器官匀浆接种小鼠更敏感的方法组合（与荧光抗体技术和补体结合试验联合共培养、器官碎片外植）才能检测到。在缓解期的慢性脑炎猴子的中枢神经系统和内脏器官中，在第90天通过相同方法检测到TBE病毒。
