Vijayakumar Sekar, Vaseeharan Baskaralingam, Malaikozhundan Balasubramanian, Gobi Narayanan, Ravichandran Samuthirapandian, Karthi Sellamuthu, Ashokkumar Balasubramaniem, Sivakumar Natesan
Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science Campus 6(th) Floor, Alagappa University, Karaikudi, 630004, Tamil Nadu, India.
Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science Campus 6(th) Floor, Alagappa University, Karaikudi, 630004, Tamil Nadu, India.
Microb Pathog. 2017 Sep;110:140-151. doi: 10.1016/j.micpath.2017.06.029. Epub 2017 Jun 23.
In the present study, we prepared fucoidan coated Au-NPs (Fu-AuNPs), and examined its antimicrobial activity against Aeromonas hydrophila. The green synthesized Fu-AuNPs were bio-physically characterized by Ultraviolet-visible (UV-Vis) spectroscopy, X-ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Higher Transmission Electron Microscopy (HR-TEM), Zeta potential analysis and Energy Dispersive X-ray spectroscopy (EDX). Fu-AuNPs were crystalline in nature, spherical to triangular in shape, with particle size ranging within 10-100 nm. The synthesized Fu-AuNPs at 100 μg mL showed inhibition zone against A. hydrophila (23.2 mm) which is much higher than that of commercial antibiotic chloramphenicol (17.3 mm). The biofilm inhibitory activity of Fu-AuNPs against Gram negative (Aeromonas hydrophila) was higher. Light and confocal laser scanning microscopic observations showed that the Fu-AuNPs at 100 μg mL inhibited the biofilm of A. hydrophila. The cytotoxicity study indicated that Fu-AuNPs were effective in inhibiting the viability of human cervical cancer cells (HeLa) at 100 μg mL. In another experiment, the antibacterial effect of Fu-AuNPs on tilapia, Oreochromis mossambicus were evaluated in vivo. The mortality rate of O. mossambicus infected by A. hydrophila was much higher (90%), whereas, the mortality of O. mossambicus that received Fu-AuNPs followed by challenge with A. hydrophia was reduced to 30%. This study concludes that Fu-AUNPs are effective in the control of A. hydrophila infections in O. mossambicus.
在本研究中，我们制备了岩藻依聚糖包覆的金纳米颗粒（Fu-AuNPs），并检测了其对嗜水气单胞菌的抗菌活性。通过紫外可见（UV-Vis）光谱、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、高分辨率透射电子显微镜（HR-TEM）、zeta电位分析和能量色散X射线光谱（EDX）对绿色合成的Fu-AuNPs进行了生物物理表征。Fu-AuNPs本质上是晶体，形状为球形至三角形，粒径在10-100nm范围内。合成的Fu-AuNPs在浓度为100μg/mL时对嗜水气单胞菌表现出抑菌圈（23.2mm），远高于市售抗生素氯霉素（17.3mm）。Fu-AuNPs对革兰氏阴性菌（嗜水气单胞菌）的生物膜抑制活性更高。光学和共聚焦激光扫描显微镜观察表明，浓度为100μg/mL的Fu-AuNPs抑制了嗜水气单胞菌的生物膜形成。细胞毒性研究表明，Fu-AuNPs在浓度为100μg/mL时能有效抑制人宫颈癌细胞（HeLa）的活力。在另一项实验中，在体内评估了Fu-AuNPs对罗非鱼（奥利亚罗非鱼）的抗菌效果。被嗜水气单胞菌感染的奥利亚罗非鱼死亡率很高（90%），而预先接受Fu-AuNPs处理后再用嗜水气单胞菌攻击的奥利亚罗非鱼死亡率降至30%。本研究得出结论，Fu-AUNPs可有效控制奥利亚罗非鱼中的嗜水气单胞菌感染。
