循环微RNA作为乙肝病毒感染持续存在的潜在生物标志物
Circulating microRNAs as potential biomarkers of HBV infection persistence.
作者信息
Chen Jie, Wu Xiaojuan, Cai Bei, Su Zhenzhen, Li Lixin, An Yunfei, Wang Lanlan
机构信息
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, PR China.
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, PR China.
出版信息
Infect Genet Evol. 2017 Oct;54:152-157. doi: 10.1016/j.meegid.2017.06.023. Epub 2017 Jun 23.
OBJECTIVES
To investigate different microRNA (miRNA) expression profile in patients of hepatitis B virus (HBV) infection compared with virus spontaneous clearance ones, and try to find new biomarker for diagnosis and treatment assessment.
METHODS
miRNA expression profiling was analyzed in patients with HBV infection and patients with virus spontaneous clearance by Affymetrix Gene Chip miRNA 4.0 Arrays. The differently expressed miRNAs, as verified by RT-PCR, were analyzed and compared with each other in the two groups of patients.
RESULTS
Significant difference was found in the expression of miR-29b, miR-34, miR-4485, miR-3180, miR-125, miR-330-3p, miR-1468 in spontaneous clearance group and CHB group. miR-29b, miR-34, miR-4485 was significantly increased in CHB group, while miR-125, miR-330-3p, miR-1468, miR-3180 was significantly decreased. Correlation analysis indicate only miR-29b was positive correlation with HBsAg.
CONCLUSIONS
These results indicated above miRNAs may be associated with HBV infection persistence, and provided us new ideas for finding potential biomarkers.
目的
研究乙肝病毒（HBV）感染患者与病毒自发清除者不同的微小RNA（miRNA）表达谱，试图寻找诊断和治疗评估的新生物标志物。
方法
采用Affymetrix Gene Chip miRNA 4.0芯片对HBV感染患者和病毒自发清除患者的miRNA表达谱进行分析。经逆转录聚合酶链反应（RT-PCR）验证的差异表达miRNA，在两组患者中进行分析和相互比较。
结果
在自发清除组和慢性乙型肝炎（CHB）组中，发现miR-29b、miR-34、miR-4485、miR-3180、miR-125、miR-330-3p、miR-1468的表达存在显著差异。CHB组中miR-29b、miR-34、miR-4485显著升高，而miR-125、miR-330-3p、miR-1468、miR-3180显著降低。相关性分析表明只有miR-29b与乙肝表面抗原（HBsAg）呈正相关。
结论
上述结果表明这些miRNA可能与HBV感染持续存在有关，并为寻找潜在生物标志物提供了新思路。
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