微小RNA-330-3p通过靶向丝裂原活化蛋白激酶激酶1抑制肝癌细胞迁移。
miR-330-3p suppresses liver cancer cell migration by targeting MAP2K1.
作者信息
Jin Zhe, Jia Baoxing, Tan Ludong, Liu Yahui
机构信息
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
出版信息
Oncol Lett. 2019 Jul;18(1):314-320. doi: 10.3892/ol.2019.10280. Epub 2019 Apr 24.
MicroRNAs, considered as a promising focus for the treatment of tumors, are key regulators of a large number of genes. The aim of the present study was to investigate the biological functions of microRNA (miR)-330-3p in liver cancer as it had been identified previously that miR-330-3p was deregulated in liver cancer. In order to identify the function of miR-330-3p in liver cancer, the expression of miR-330-3p was determined in liver cancer tissues and adjacent non-tumor tissues using reverse transcription-quantitative polymerase chain reaction analysis. To elucidate the function of miR-330-3p in liver cancer, miR-330-3p was overexpressed using mimic transfection. Cell migration was inhibited by miR-330-3p in liver cancer cells. The miRNA target prediction databases were used to identify potential target genes of miR-330-3p in liver cancer. The RNA level of mitogen-activated protein kinase kinase 1 (MAP2K1) was downregulated by miR-330-3p in liver cancer cells. In conclusion, miR-330-3p suppresses cell migration by targeting MAP2K1 in liver cancer cells.
微小RNA被认为是肿瘤治疗的一个有前景的研究重点，是大量基因的关键调节因子。本研究的目的是探讨微小RNA（miR）-330-3p在肝癌中的生物学功能，因为先前已发现miR-330-3p在肝癌中表达失调。为了确定miR-330-3p在肝癌中的功能，采用逆转录-定量聚合酶链反应分析方法检测肝癌组织及癌旁非肿瘤组织中miR-330-3p的表达。为了阐明miR-330-3p在肝癌中的功能，通过模拟转染使miR-330-3p过表达。miR-330-3p抑制肝癌细胞的迁移。利用微小RNA靶标预测数据库鉴定肝癌中miR-330-3p的潜在靶基因。在肝癌细胞中，miR-330-3p下调丝裂原活化蛋白激酶激酶1（MAP2K1）的RNA水平。总之，miR-330-3p通过靶向肝癌细胞中的MAP2K1抑制细胞迁移。
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