Zhang Hao, Sun Lingli, Wang Huaiming, Cai Huan, Niu Guozhong, Bai Yongjie, Zhang Yun, Yang Dong, Gu Mengmeng, Xu Pengfei, Fan Xinying, Liu Xinfeng, Xu Gelin
Department of Neurology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China; Department of Neurology, The First People's Hospital of Hangzhou, Nanjing Medical University, Hangzhou, Zhejiang 310006, China.
Department of Neurology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, China.
J Stroke Cerebrovasc Dis. 2017 Oct;26(10):2294-2299. doi: 10.1016/j.jstrokecerebrovasdis.2017.05.013. Epub 2017 Jun 23.
The variant rs9943582 of APLNR (apelin receptor) was identified by a large-scale study to be associated with an increased risk of ischemic stroke in a Japanese population. We conducted this study to investigate the association between the variant and age of onset and clinical outcomes of ischemic stroke in a Chinese population.
Improved multiple ligase detection reaction was used to genotype the variant. We compared the mean age at ischemic stroke onset with one-way ANOVA. The Kaplan-Meier method, log-rank test, and Cox proportional hazards regression models were performed to analyze the association between the variant and clinical outcomes (recurrence and death).
A total of 916 ischemic stroke patients were recruited for the study. For age at ischemic stroke onset, no significant association was identified with the variant in any genetic model. In addition, the variant was not strongly associated with recurrence and death risk of ischemic stroke, as shown by the results.
The findings indicated that the variant rs9943582 was not associated with age at onset and clinical outcomes of ischemic stroke. However, evidence from well-designed studies with larger and in different ethnic populations are warranted to further explore the effects of APLNR on the ischemic stroke onset and clinical outcomes.
通过一项大规模研究发现，APLNR（apelin受体）的rs9943582变异与日本人群缺血性中风风险增加有关。我们开展本研究以调查该变异与中国人群缺血性中风发病年龄及临床结局之间的关联。
采用改良多重连接酶检测反应对该变异进行基因分型。我们用单因素方差分析比较缺血性中风发病的平均年龄。采用Kaplan-Meier法、对数秩检验和Cox比例风险回归模型分析该变异与临床结局（复发和死亡）之间的关联。
本研究共纳入916例缺血性中风患者。对于缺血性中风发病年龄，在任何遗传模型中均未发现该变异与之有显著关联。此外，结果显示该变异与缺血性中风的复发和死亡风险也无强关联。
研究结果表明，rs9943582变异与缺血性中风的发病年龄及临床结局无关。然而，需要来自设计良好、样本量更大且不同种族人群研究的证据，以进一步探究APLNR对缺血性中风发病及临床结局的影响。
