Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Laboratório de Microbiologia de Leite, Departamento de Tecnologia e Inspeção de Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Transbound Emerg Dis. 2018 Feb;65(1):e40-e47. doi: 10.1111/tbed.12666. Epub 2017 Jun 26.
Vaccinia virus (VACV) is the agent of bovine vaccinia (BV), an emerging zoonosis that causes exanthematic lesions on the teats of dairy cows and on the hands of milkers. The virus has been detected in the milk of naturally infected cows. The objective of this study was to investigate and quantify VACV DNA as well as the presence of infectious virus particles in samples of cheese curd, cheese whey and pasteurized milk produced using milk from cows experimentally inoculated with VACV-GP2, a Brazilian isolate of VACV (VACV-BR). VACV DNA was detected in samples of cheese and pasteurized milk at different time points, even after the resolution of the typical lesions caused by VACV, which occurred after 22 days post-infection (dpi), on average. Moreover, it was possible to detect infectious viral particles in cheese samples on alternate days until 27 dpi. The presence of both VACV DNA and infectious viral particles in cheese samples throughout the clinical course of BV and even after the disappearance of the typical clinical signs of disease draws attention to the risk associated with consumption of the cheese. Furthermore, VACV-contaminated milk and cheese may represent an occupational risk to cheesemakers who often manipulate milk and cheese curd without wearing gloves.
牛痘病毒（VACV）是牛痘（BV）的病原体，BV 是一种新兴的人畜共患病，会导致奶牛的乳头和挤奶工的手部出现疹状病变。该病毒已在自然感染奶牛的牛奶中被检测到。本研究的目的是调查和定量 VACV DNA 以及在使用感染 VACV-GP2 的奶牛的牛奶生产的干酪凝乳、奶酪乳清和巴氏杀菌奶样品中是否存在传染性病毒颗粒，VACV-GP2 是巴西分离株 VACV（VACV-BR）。在不同时间点的奶酪和巴氏杀菌奶样品中均检测到 VACV DNA，甚至在感染后 22 天（平均）出现由 VACV 引起的典型病变消退后也是如此。此外，在 27 天的感染后，每隔一天就可以在奶酪样本中检测到传染性病毒颗粒。在 BV 的整个临床过程中，甚至在典型临床症状消失后，在奶酪样本中都能同时检测到 VACV DNA 和传染性病毒颗粒，这引起了对食用奶酪相关风险的关注。此外，受 VACV 污染的牛奶和奶酪可能会对经常在不戴手套的情况下处理牛奶和奶酪凝乳的奶酪制造商构成职业风险。
