a Institute of Biophysics, 2nd Faculty of Medicine , Charles University , Prague , Czech Republic.
b Laboratory of Tissue Engineering, Institute of Experimental Medicine , Czech Academy of Sciences , Prague , Czech Republic.
Platelets. 2018 Jun;29(4):395-405. doi: 10.1080/09537104.2017.1316838. Epub 2017 Jun 26.
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% (v/v) platelet lysate was sufficient to supplement 10% (v/v) FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes; however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.
血小板是组织工程应用中天然生长因子的常用来源。本研究旨在验证血小板裂解液作为胎牛血清（FBS）替代物用于皮肤细胞培养的用途。使用 Bio-Plex 系统对血小板裂解液的细胞因子含量进行了表征。将细胞（成纤维细胞、黑素细胞和角质形成细胞）培养在 PCL 纳米纤维支架上，以模拟其天然微环境。测定了血小板裂解液的细胞因子含量，并向细胞中添加含有血小板裂解液或血小板裂解液与 FBS 的培养基。结果表明，7%（v/v）的血小板裂解液足以补充成纤维细胞和角质形成细胞培养物中 10%（v/v）的 FBS。血小板裂解液与 FBS 的组合对成纤维细胞具有较强的抑制作用，而对角质形成细胞则具有协同作用。血小板裂解液不能充分促进黑素细胞的增殖；然而，FBS 和血小板裂解液的组合产生了更好的结果，导致培养的黑素细胞呈现双极形态。数据表明，血小板裂解液可促进细胞增殖和代谢活性，可作为细胞培养基的添加剂。
