D'Urso A, Randazzo R, Rizzo V, Gangemi C M A, Romanucci V, Zarrelli A, Tomaselli G, Milardi D, Borbone N, Purrello R, Piccialli G, Di Fabio G, Oliviero G
Department of Chemical Science, University of Catania, V.le A Doria 6, 95125, Catania, Italy.
Phys Chem Chem Phys. 2017 Jul 14;19(26):17404-17410. doi: 10.1039/c7cp02816d. Epub 2017 Jun 26.
The interaction of the porphyrin derivative HTCPPSpm4, having spermine pendants in the four meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG has been investigated by means of UV, electronic circular dichroism and PAGE studies. The results reported here demonstrate that the porphyrin derivative is capable of stabilizing or destabilizing the higher-ordered structures of parallel GQs, depending on the method used to reach their relative stoichiometry (titration vs. single addition). Noteworthily, when two equivalents of HTCPPSpm4 were mixed directly with one equivalent of the (TGGGAG) GQ to reach a 2 : 1 HTCPPSpm4 : GQ ratio T higher than 80 °C was also observed confirming the presence of higher-ordered GQ structures.
通过紫外光谱、电子圆二色光谱和聚丙烯酰胺凝胶电泳研究，对在四个中位位置带有精胺侧链的卟啉衍生物HTCPPSpm4与由DNA适配体TGGGAG形成的G-四链体（GQ）结构之间的相互作用进行了研究。此处报道的结果表明，根据达到其相对化学计量比的方法（滴定法与单次添加法），该卟啉衍生物能够稳定或破坏平行GQ的高阶结构。值得注意的是，当将两当量的HTCPPSpm4与一当量的（TGGGAG）GQ直接混合以达到2:1的HTCPPSpm4:GQ比例时，还观察到温度高于80°C，这证实了高阶GQ结构的存在。
